Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture

被引：70

作者：

Choi, Da Jeong ^{[1
,5
]}

Choi, Seung Mi ^{[1
,5
]}

Kang, Hae Yeong ^{[1
,5
]}

Min, Hye-Jin ^{[1
,5
]}

Lee, Rira ^{[1
,5
]}

Ikram, Muhammad ^{[1
,5
]}

Subhan, Fazli ^{[1
,5
]}

Jin, Song Wan ^{[2
,5
]}

Jeong, Young Hun ^{[3
,5
]}

Kwak, Jong-Young ^{[4
,5
]}

Yoon, Sik ^{[1
,5
]}

机构：

[1] Pusan Natl Univ, Sch Med, Dept Anat, Yangsan 626870, Gyeongsangnam D, South Korea

[2] Korea Polytech Univ, Dept Mech Engn, Shihung 429793, South Korea

[3] Kyungpook Natl Univ, Dept Mech Engn, Taegu 702701, South Korea

[4] Dong A Univ, Sch Med, Dept Biochem, Busan 602714, South Korea

[5] Pioneer Res Ctr, Seoul, South Korea

来源：

JOURNAL OF BIOTECHNOLOGY | 2015年 / 205卷

基金：

新加坡国家研究基金会;

关键词：

Electrospun nanofiber; Composite scaffold; Fish collagen; Thymic epithelial cells; 3D cell culture; I COLLAGEN; PROLIFERATION; FIBROBLASTS; MIGRATION; ADHESION; GUIDANCE; GROWTH;

D O I：

10.1016/j.jbiotec.2015.01.017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

One of the most challenging objectives of 3D cell culture is the development of scaffolding materials with outstanding biocompatibility and favorable mechanical strength. In this study, we fabricated a novel nanofibrous scaffold composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibrous scaffolds were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreased as FC content was increased in the FC/PCL composite nanofibers. The cytocompatibility of the FC/PCL scaffolds was evaluated by SEM, WST-1 assay, confocal microscopy, western blot, and RT-PCR. It was found that the scaffolds not only facilitated the adhesion, spreading, protrusions, and proliferation of thymic epithelial cells (TECs), but also stimulated the expression of genes and proteins involved in cell adhesion and T-cell development. Thus, these results suggest that the FC/PCL composite nanofibrous scaffolds will be a useful model of 3D cell culture for TECs and may have wide applicability in the future for engineering tissues or organs. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：47 / 58

页数：12

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