Newly developed apparatus for inoculating plant organs into large-scale fermentor

被引：7

作者：

Kawamura, M ^{[1
]}

Shigeoka, T ^{[1
]}

Akita, M ^{[1
]}

Kobayashi, Y ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD, TSUKUBA RES LABS, PCC TECHNOL INC, TSUKUBA LAB, TSUKUBA, IBARAKI 305, JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1996年 / 82卷 / 06期

关键词：

organ culture; inoculation apparatus; large-scale fermenter;

D O I：

10.1016/S0922-338X(97)81266-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed an apparatus for inoculation of a large number of plantlets or plant parts such as shoots or roots into a large-scale fermenter at one time. Ab opa belladonna adventitious roots (2.4 kg FW) were inoculated into a 500 l fermenter under sterile conditions, and 35 kg FW of the roots were obtained after 60-d cultivation. There were no differences in proliferation rate and scopolamine content between a 10 l fermenter culture used as control and the 500 l fermenter culture. The success of large-scale root culture was considered to be dependent on the use of the new inoculation apparatus.

引用

页码：618 / 619

页数：2

共 7 条

[1]

AKITA M, 1994, PLANT CELL REP, V13, P180, DOI 10.1007/BF00239888

[2]

KOBAYASHI Y, 1993, APPL MICROBIOL BIOT, V40, P215, DOI 10.1007/BF00170369

[3] THE PRODUCTION OF SECONDARY METABOLITES BY PLANT-CELLS CULTIVATED IN BIOREACTORS [J].