Achieving differentiation of single-base mutations through hairpin oligonucleotide and electric potential control

被引：46

作者：

Wei, F ^{[1
]}

Sun, B ^{[1
]}

Liao, W ^{[1
]}

Ouyang, JH ^{[1
]}

Zhao, XS ^{[1
]}

机构：

[1] Peking Univ, Dept Biol Chem, Coll Chem, State Key Lab Mol Dynam & Stable Struct, Beijing 100871, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 09期

基金：

中国国家自然科学基金;

关键词：

biosensor; hairpin oligonucleotide; electric potential; gene chip; impedance; single-base mutation;

D O I：

10.1016/S0956-5663(02)00249-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel assay for surface DNA hybridization, which is free of sample and probe labeling, convenient and of low cost, sensitive and capable of differentiation of single-base mutations, is reported. Hairpin oligonucleotides are carefully designed as probes and are covalently attached to Si chips. Segments of the human p53 gene are chosen to demonstrate the major features of the novel technique. Impedance measurement is used to detect the hybridization. To further optimize the performance, electric potential is applied on the chip. The apparently different responses of the chip to the complementary strand and the single-base mutant are shown under electric potential control. The criteria on the design of the hairpin oligonucleotides are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1149 / 1155

页数：7

共 29 条

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