cDNA cloning of mouse BLM gene, the homologue to human Bloom's syndrome gene, which is highly expressed in the testis at the mRNA level

被引：21

作者：

Seki, T

Wang, WS

Okumura, N

Seki, M

Katada, T

Enomoto, T ^{[1
]}

机构：

[1] Tohoku Univ, Fac Pharmaceut Sci, Dept Mol Cell Biol, Sendai, Miyagi 9808578, Japan

[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Physiol Chem, Tokyo 1130033, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1398卷 / 03期

基金：

日本学术振兴会;

关键词：

Bloom's syndrome; expression in the testis; pachytene; (mouse);

D O I：

10.1016/S0167-4781(98)00066-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a cDNA encoding the mouse homologue to human Bloom's syndrome gene (BLM). The deduced amino acid sequence of mouse Blm showed 76% identity to the human sequence with very high homology in seven consecutive domains characteristic of DNA and RNA helicases. The expression of mBLM mRNA was examined in various tissues. Extremely high expression was observed in the testis as compared with other tissues. The mBLM mRNA level in the testis began to increase 12-14 days after birth, corresponding to the appearance of cells in the pachytene phase. (C) 1998 Elsevier Science B.V. All rights reserved.

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页码：377 / 381

页数：5

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