Glucose-sensitive ion-sensitive field-effect transistor-based biosensor with additional positively charged membrane - Dynamic range extension and reduction of buffer concentration influence on the sensor response

被引:40
作者
Volotovsky, V
Soldatkin, AP
Shulga, AA
Rossokhaty, VK
Strikha, VI
Elskaya, AV
机构
[1] NATL ACAD SCI UKRAINE, INST MOLEC BIOL & GENET, KIEV 252143, UKRAINE
[2] KIEV TG SHEVCHENKO STATE UNIV, SECTOR BIOELECTR, KIEV 252001, UKRAINE
[3] INST CHEM & BIOSENSOR, D-48149 MUNSTER, GERMANY
关键词
biosensors; enzymatic methods; ISFETs; glucose; dynamic range; buffer capacity; membranes;
D O I
10.1016/0003-2670(95)00592-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Glucose-sensitive sensors based on ion-sensitive field-effect transistors were prepared by cross-linking a mixture of glucose oxidase and bovine serum albumin in saturated glutaraldehyde vapour on the sensitive area of the transducer. After this procedure, 4-vinylpyridine-styrene copolymer membranes were deposited on top of the sensors by drop-coating of 0.5% polymer solution in ethanol. The use of this additional membrane resulted in substantial extension of the dynamic range of the sensor (up to 20 mM of glucose) and reduction of buffer concentration dependence of the sensor response. Also the effects of pH and ionic strength were examined for the sensors with and without additional membranes.
引用
收藏
页码:77 / 81
页数:5
相关论文
共 7 条
  • [1] PEREDERIJ VG, 1993, CLIN ESTIMATION BIOC, P58
  • [2] IMPLANTABLE GLUCOSE SENSORS - CHOOSING THE APPROPRIATE SENSING STRATEGY
    PICKUP, JC
    SHAW, GW
    CLAREMONT, DJ
    [J]. BIOSENSORS, 1988, 3 (06) : 335 - 346
  • [3] REMOVING THE INFLUENCE OF BUFFER CONCENTRATION ON THE RESPONSE OF ENZYME FIELD-EFFECT TRANSISTORS BY USING ADDITIONAL MEMBRANES
    SHULGA, AA
    STRIKHA, VI
    SOLDATKIN, AP
    ELSKAYA, AV
    MAUPAS, H
    MARTELET, C
    CLECHET, P
    [J]. ANALYTICA CHIMICA ACTA, 1993, 278 (02) : 233 - 236
  • [4] OVERALL CHARACTERIZATION OF ISFET-BASED GLUCOSE BIOSENSOR
    SHULGA, AA
    SANDROVSKY, AC
    STRIKHA, VI
    SOLDATKIN, AP
    STARODUB, NF
    ELSKAYA, AV
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 1992, 10 (01) : 41 - 46
  • [5] SHULGA AA, 1995, IN PRESS SENSORS ACT
  • [6] GLUCOSE-SENSITIVE CONDUCTOMETRIC BIOSENSOR WITH ADDITIONAL NAFION MEMBRANE - REDUCTION OF INFLUENCE OF BUFFER CAPACITY ON THE SENSOR RESPONSE AND EXTENSION OF ITS DYNAMIC-RANGE
    SOLDATKIN, AP
    ELSKAYA, AV
    SHULGA, AA
    JDANOVA, AS
    DZYADEVICH, SV
    JAFFREZICRENAULT, N
    MARTELET, C
    CLECHET, P
    [J]. ANALYTICA CHIMICA ACTA, 1994, 288 (03) : 197 - 203
  • [7] GLUCOSE-SENSITIVE FIELD-EFFECT TRANSISTOR WITH ADDITIONAL NAFION MEMBRANE - REDUCTION OF INFLUENCE OF BUFFER CAPACITY ON THE SENSOR RESPONSE AND EXTENSION OF ITS DYNAMIC-RANGE
    SOLDATKIN, AP
    ELSKAYA, AV
    SHULGA, AA
    NETCHIPOROUK, LI
    HENDJI, AMN
    JAFFREZICRENAULT, N
    MARTELET, C
    [J]. ANALYTICA CHIMICA ACTA, 1993, 283 (02) : 695 - 701