Paracrine mitogenic effect of human endothelial progenitor cells: role of interleukin-8

被引：74

作者：

He, TR

Peterson, TE

Katusic, ZS

机构：

[1] Mayo Clin, Coll Med, Dept Mol Pharmacol & Expt Therapeut, Rochester, MN 55905 USA

[2] Mayo Clin, Coll Med, Dept Anesthesiol, Rochester, MN 55905 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2005年 / 289卷 / 02期

关键词：

angiogenesis; neovascularization; cytokines;

D O I：

10.1152/ajpheart.01166.2004

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Endothelial progenitor cells (EPCs) play an important role in repair of vascular injury and neovascularization. Molecular mechanisms underlying vascular effects of EPCs are not fully understood. The present study was designed to test the hypothesis that human EPCs exert a strong paracrine mitogenic effect on mature endothelial cells. Levels of interleukin-8 (IL-8) were significantly higher in conditioned medium (CM) collected from EPCs than in CM derived from mature endothelial cells [umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (CAECs)]. CM of EPCs stimulated proliferation of HUVECs and CAECs. This mitogenic effect was partially inhibited by IL-8-neutralizing antibody. In contrast, CM of HUVECs and CAECs had a weak or no mitogenic effect on mature endothelial cells. Our results demonstrate significantly higher levels of IL-8 secretion by human EPCs than by mature endothelial cells. IL-8 appears to be an important mediator of the paracrine mitogenic effect of EPCs.

引用

收藏

页码：H968 / H972

页数：5

相关论文

共 21 条

[1] Isolation of putative progenitor endothelial cells for angiogenesis [J].

Asahara, T ;

Murohara, T ;

Sullivan, A ;

Silver, M ;

vanderZee, R ;

Li, T ;

Witzenbichler, B ;

Schatteman, G ;

Isner, JM .

SCIENCE, 1997, 275 (5302) :964-967

[2] Altered angiogenesis and survival in human tumor-derived endothelial cells [J].

Bussolati, B ;

Deambrosis, I ;

Russo, S ;

Deregibus, MC ;

Camussi, G .

FASEB JOURNAL, 2003, 17 (06) :1159-+

[3] Paracrine and autocrine effects of fibroblast growth factor-4 in endothelial cells [J].

Dell'Era, P ;

Belleri, M ;

Stabile, H ;

Massardi, ML ;

Ribatti, D ;

Presta, M .

ONCOGENE, 2001, 20 (21) :2655-2663

[4]

Gualandris A, 1996, CELL GROWTH DIFFER, V7, P147

[5] Modulation of the vascular response to injury by autologous blood-derived outgrowth endothelial cells [J].

Gulati, R ;

Jevremovic, D ;

Witt, TA ;

Kleppe, LS ;

Vile, RG ;

Lerman, A ;

Simari, RD .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2004, 287 (02) :H512-H517

[6] Human endothelial progenitor cells tolerate oxidative stress due to intrinsically high expression of manganese superoxide dismutase [J].

He, TR ;

Peterson, TE ;

Holmuhamedov, EL ;

Terzic, A ;

Caplice, NM ;

Oberley, LW ;

Katusic, ZS .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (11) :2021-2027

[7] Transplantation of circulating endothelial progenitor cells restores endothelial function of denuded rabbit carotid arteries [J].

He, TR ;

Smith, LA ;

Harrington, S ;

Nath, KA ;

Caplice, NM ;

Katusic, ZS .

STROKE, 2004, 35 (10) :2378-2384

[8] Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis [J].

Hur, J ;

Yoon, CH ;

Kim, HS ;

Choi, JH ;

Kang, HJ ;

Hwang, KK ;

Oh, BH ;

Lee, MM ;

Park, YB .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (02) :288-293

[9] Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization [J].

Kalka, C ;

Masuda, H ;

Takahashi, T ;

Kalka-Moll, WM ;

Silver, M ;

Kearney, M ;

Li, T ;

Isner, JM ;

Asahara, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3422-3427

[10] Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms [J].

Kinnaird, T ;

Stabile, E ;

Burnett, MS ;

Lee, CW ;

Barr, S ;

Fuchs, S ;

Epstein, SE .

CIRCULATION RESEARCH, 2004, 94 (05) :678-685