Complete genome sequence of the Lactococcus lactis temperate phage φLC3:: comparative analysis of φLC3 and its relatives in lactococci and streptococci

被引:25
作者
Blatny, JM [1 ]
Godager, L [1 ]
Lunde, M [1 ]
Nes, IF [1 ]
机构
[1] Agr Univ Norway, Dept Chem & Biotechnol, Lab Micrbial Gene Technol, N-1432 As, Norway
关键词
temperate; bacteriophage; genome sequence; Lactococcus lactis; Streptococcus pyogenes; comparative analysis; genetic exchange;
D O I
10.1016/j.virol.2003.09.019
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Complete genome sequencing of the P335 temperate Lactococcus lactis bacteriophage phiLC3 (32, 172 bp) revealed fifty-one open reading frames (ORFs). Four ORFs did not show any homology to other proteins in the database and twenty-one ORFs were assigned a putative biological function. phiLC3 contained a unique replication module and orf201 was identified as the putative replication initiator protein-encoding gene. phiLC3 was closely related to the L. lactis rlt phage (73% DNA identity). Similarity was also shared with other lactococcal P335 phages and the Streptococcus pyogenes prophages 370.3, 8232.4 and 315.5 over the non-structural genes and the genes involved in DNA packaging/phage morphogenesis, respectively. phiLC3 contained small homologous regions distributed among lactococcal phages suggesting that these regions might be involved in mediating genetic exchange. Two regions of 30 and 32 bp were conserved among the streptococcal and lactococcal rlt-like phages. These two regions, as well as other homologous regions, were located at mosaic borders and close to putative transcriptional terminators indicating that such regions together might attract recombination. The conserved regions found among lactococcal and streptococcal phages might be used for identification of phages/prophages/prophage remnants in their hosts. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:231 / 244
页数:14
相关论文
共 56 条
[1]  
Ackermann HW, 1999, ADV VIRUS RES, V51, P135
[2]   Genome sequence of a serotype M3 strain of group A Streptococcus:: Phage-encoded toxins, the high-virulence phenotype, and clone emergence [J].
Beres, SB ;
Sylva, GL ;
Barbian, KD ;
Lei, BF ;
Hoff, JS ;
Mammarella, ND ;
Liu, MY ;
Smoot, JC ;
Porcella, SF ;
Parkins, LD ;
Campbell, DS ;
Smith, TM ;
McCormick, JK ;
Leung, DYM ;
Schlievert, PM ;
Musser, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (15) :10078-10083
[3]   CLONING, MOLECULAR CHARACTERIZATION, AND EXPRESSION OF THE GENES ENCODING THE LYTIC FUNCTIONS OF LACTOCOCCAL BACTERIOPHAGE-PHI-LC3 - A DUAL LYSIS SYSTEM OF MODULAR DESIGN [J].
BIRKELAND, NK .
CANADIAN JOURNAL OF MICROBIOLOGY, 1994, 40 (08) :658-665
[4]   Transcriptional analysis of the genetic elements involved in the lysogeny/lysis switch in the temperate lactococcal bacteriophage φLC3, and identification of the Cro-like protein ORF76 [J].
Blatny, JM ;
Ventura, M ;
Rosenhaven, EM ;
Risoen, PA ;
Lunde, M ;
Brüssow, H ;
Nes, IF .
MOLECULAR GENETICS AND GENOMICS, 2003, 269 (04) :487-498
[5]   Analysis of a regulator involved in the genetic switch between lysis and lysogeny of the temperate Lactococcus lactis phage φLC3 [J].
Blatny, JM ;
Risoen, PA ;
Lillehaug, D ;
Lunde, M ;
Nes, IF .
MOLECULAR GENETICS AND GENOMICS, 2001, 265 (01) :189-197
[6]  
Botstein D, 1980, Ann N Y Acad Sci, V354, P484, DOI 10.1111/j.1749-6632.1980.tb27987.x
[7]   Homologous recombination between a lactococcal bacteriophage and the chromosome of its host strain [J].
Bouchard, JD ;
Moineau, S .
VIROLOGY, 2000, 270 (01) :65-75
[8]   Phage genomics:: Small is beautiful [J].
Brüssow, H ;
Hendrix, RW .
CELL, 2002, 108 (01) :13-16
[9]   Comparative phage genomics and the evolution of Siphoviridae:: insights from dairy phages [J].
Brüssow, H ;
Desiere, F .
MOLECULAR MICROBIOLOGY, 2001, 39 (02) :213-222
[10]   Phages of dairy bacteria [J].
Brüssow, H .
ANNUAL REVIEW OF MICROBIOLOGY, 2001, 55 :283-303