Cluster morphology of protein-coated polymer colloids

被引:14
作者
Molina-Bolívar, JA [1 ]
Galisteo-González, F [1 ]
Hidalgo-Alvarez, R [1 ]
机构
[1] Univ Granada, Fac Ciencias, Dept Fis Aplicada, Grp Fis Fluidos & Biocoloides, E-18071 Granada, Spain
关键词
polystyrene-protein systems; static light scattering; fractal structure; reversible aggregation; restructuring;
D O I
10.1006/jcis.1998.5831
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We report measurements on the aggregation processes in a colloidal suspension of polystyrene particles covered with F(ab')(2) (immunoglobulin IgG fragment) performed by static and dynamic light scattering. In order to study the cluster morphology of aggregates, the fractal dimension is obtained from the dependence of the scattered intensity on the scattering wave number. The stability domains of bare and protein-coated polystyrene particles were examined by plotting the stability ratio as a function of electrolyte concentration. The stability results have been explained using a modified Dejaguin-Landau-Verwey-Overbeek theory to describe the interparticle interaction. The observed change in the fractal dimension can be explained by the existence of a minimum separation distance between coagulated particles (restructuring). This minimum distance is attributed to the layer of hydrated ions and water molecules adsorbed on the particle surface. Our results are in agreement with the reversible-growth model of W. Y. Shih, I. A. Aksay, and R. Kikuchi (Phys. Rev. A. 36, 5015 (1987)) and they were supported by transmission electron microscopy observation. (C) 1998 Academic Press.
引用
收藏
页码:445 / 454
页数:10
相关论文
共 67 条
[1]   FRACTAL STRUCTURE OF HEMATITE AGGREGATES [J].
AMAL, R ;
RAPER, JA ;
WAITE, TD .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1990, 140 (01) :158-168
[2]   RESTRUCTURING OF COLLOIDAL SILICA AGGREGATES [J].
AUBERT, C ;
CANNELL, DS .
PHYSICAL REVIEW LETTERS, 1986, 56 (07) :738-741
[3]   SEX-DIFFERENCES IN THE EXPRESSION OF DEPRESSION IN ADOLESCENTS [J].
BARON, P ;
JOLY, E .
SEX ROLES, 1988, 18 (1-2) :1-7
[4]   KINETICS OF SALT-INDUCED AGGREGATION IN POLYSTYRENE LATTICES STUDIED BY QUASI-ELASTIC LIGHT-SCATTERING [J].
BOLLE, G ;
CAMETTI, C ;
CODASTEFANO, P ;
TARTAGLIA, P .
PHYSICAL REVIEW A, 1987, 35 (02) :837-841
[5]   Using phase transitions to investigate the effect of salts on protein interactions [J].
Broide, ML ;
Tominc, TM ;
Saxowsky, MD .
PHYSICAL REVIEW E, 1996, 53 (06) :6325-6335
[6]   SALT-INDUCED FAST AGGREGATION OF POLYSTYRENE LATEX [J].
CARPINETI, M ;
FERRI, F ;
GIGLIO, M ;
PAGANINI, E ;
PERINI, U .
PHYSICAL REVIEW A, 1990, 42 (12) :7347-7354
[7]  
COLOWICK SP, 1987, METHODS ENZYMOLOGY, V136
[8]  
CZARNECKI J, 1980, J COLLOID INTERF SCI, V78, P29
[9]  
Derjaguin BV., 1941, ACTA PHYSICOCHIM URS, V14, P633, DOI DOI 10.1016/0079-6816(93)90013-L
[10]   STRUCTURE OF AGGREGATED GOLD COLLOIDS [J].
DIMON, P ;
SINHA, SK ;
WEITZ, DA ;
SAFINYA, CR ;
SMITH, GS ;
VARADY, WA ;
LINDSAY, HM .
PHYSICAL REVIEW LETTERS, 1986, 57 (05) :595-598