Purification of histidine tagged bacteriorhodopsin, pharaonis halorhodopsin and pharaonis sensory rhodopsin II functionally expressed in Escherichia coli

被引：115

作者：

Hohenfeld, IP ^{[1
]}

Wegener, AA ^{[1
]}

Engelhard, M ^{[1
]}

机构：

[1] Max Planck Inst Mol Physiol, D-44139 Dortmund, Germany

来源：

FEBS LETTERS | 1999年 / 442卷 / 2-3期

关键词：

bacterial rhodopsin; phoborhodopsin; photocycle; eubacterial expression; mass spectrometry; immunogold labelling;

D O I：

10.1016/S0014-5793(98)01659-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteriorhodopsin (BR) from Halobacterium salinarum as well as halorhodopsin (pHR) and sensory rhodopsin II (pSRII) from Natronobacterium pharaonis were functionally expressed in E. coli using the method of Shimono et al, [FEBS Lett, (1997) 420. 54-56]. The histidine tagged proteins were purified with yields up to 1.0 mg/l cell culture and characterized by ESI mass spectrometry and their photocycle. The pSRII and PHR photocycles were indistinguishable from the wild type proteins. The BR photocycle was considerably prolonged. pSOII is located in the cytoplasmic membrane and the C-terminus is oriented ton ards the cytoplasm as determined by immunogold labelling. (C) 1999 Federation of European Biochemical Societies.

引用

页码：198 / 202

页数：5

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