cse15, cse60, and csk22 are new members of mother-cell-specific sporulation regulons in Bacillus subtilis

被引:17
作者
Henriques, AO
Bryan, EM
Beall, BW
Moran, CP
机构
[1] EMORY UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, ATLANTA, GA 30322 USA
[2] UNIV NOVA LISBOA, INST TECNOL QUIM & BIOL, P-2780 OEIRAS, PORTUGAL
关键词
PENICILLIN-BINDING PROTEIN; SIGMA-29; RNA-POLYMERASE; GENE-EXPRESSION; SPORE COAT; TRANSCRIPTION FACTOR; RESISTANCE GENE; SPATIAL CONTROL; PROMOTER; CLONING; OPERON;
D O I
10.1128/jb.179.2.389-398.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requirement for sigma(E). Maximal expression of both cse15 and cse60 further depended on the DNA-binding protein SpoIIID. Primer extension results revealed -10 and -35 sequences upstream of the cse15 and cse60 coding sequences very similar to those utilized by sigma(E)-containing RNA polymerase. Alignment of these and other regulatory regions led to a revised consensus sequence for sigma(E)-dependent promoters. A third transcriptional unit, designated csk22, was localized at approximately 173 degrees on the chromosome. Transcription of csk22 was activated at h 4 of sporulation, required the late mother-cell regulator sigma(K), and was repressed by the GerE protein. Sequences in the csk22 promoter region were similar to those of other sigma(K)-dependent promoters. The cse60 locus was deduced to encode an acidic product of only 60 residues. A 37.6-kDa protein apparently encoded by cse15 was weakly related to the heavy chain of myosins, as well as to other myosin-like proteins, and is predicted to contain a central, 100 residue-long coiled-coil domain. Finally, csk22 is inferred to encode a 18.2-kDa hydrophobic product with five possible membrane-spanning helices, which could function as a transporter.
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页码:389 / 398
页数:10
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