Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis

被引：62

作者：

Geluk, A

van Meijgaarden, KE

Franken, KLMC

Wieles, B

Arend, SM

Faber, WR

Naafs, B

Ottenhoff, THM

机构：

[1] Leiden Univ, Med Ctr, Dept Immunohematol & Blood Transfus, NL-2300 RC Leiden, Netherlands

[2] Leiden Univ, Med Ctr, Dept Dermatol, NL-2300 RC Leiden, Netherlands

[3] Leiden Univ, Med Ctr, Dept Infect Dis, NL-2300 RC Leiden, Netherlands

[4] Amsterdam Med Ctr, Dept Dermatol, Amsterdam, Netherlands

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 2004年 / 59卷 / 01期

关键词：

D O I：

10.1111/j.0300-9475.2004.01358.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guerin (BCG) strains but present in M. leprae, M tuberculosis, M bovis, M kansasii, M africanum and M marinum. In this study, the homologue of CFP-10 in M leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M leprae is a potent antigen that crossreacts with CFP-10 of M tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases.

引用

页码：66 / 70

页数：5

共 16 条

[1] EFFECTIVE VACCINATION OF MICE AGAINST MYCOBACTERIUM-TUBERCULOSIS INFECTION WITH A SOLUBLE MIXTURE OF SECRETED MYCOBACTERIAL PROTEINS [J].

ANDERSEN, P .

INFECTION AND IMMUNITY, 1994, 62 (06) :2536-2544

[2] Detection of active tuberculosis infection by T cell responses to early-secreted antigenic target 6-kDa protein and culture filtrate protein 10 [J].

Arend, SM ;

Andersen, P ;

van Meijgaarden, KE ;

Skjot, RLV ;

Subronto, YW ;

van Dissel, JT ;

Ottenhoff, THM .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (05) :1850-1854

[3] Evaluation of new vaccines in the mouse and guinea pig model of tuberculosis [J].

Baldwin, SL ;

D'Souza, C ;

Roberts, AD ;

Kelly, BP ;

Frank, AA ;

Lui, MA ;

Ulmer, JB ;

Huygen, K ;

McMurray, DM ;

Orme, IM .

INFECTION AND IMMUNITY, 1998, 66 (06) :2951-2959

[4] A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10) [J].

Berthet, FX ;

Rasmussen, PB ;

Rosenkrands, I ;

Andersen, P ;

Gicquel, B .

MICROBIOLOGY-UK, 1998, 144 :3195-3203

[5] Massive gene decay in the leprosy bacillus [J].

Cole, ST ;

Eiglmeier, K ;

Parkhill, J ;

James, KD ;

Thomson, NR ;

Wheeler, PR ;

Honoré, N ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Mungall, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, RM ;

Devlin, K ;

Duthoy, S ;

Feltwell, T ;

Fraser, A ;

Hamlin, N ;

Holroyd, S ;

Hornsby, T ;

Jagels, K ;

Lacroix, C ;

Maclean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Quail, MA ;

Rajandream, MA ;

Rutherford, KM ;

Rutter, S ;

Seeger, K ;

Simon, S ;

Simmonds, M ;

Skelton, J ;

Squares, R ;

Squares, S ;

Stevens, K ;

Taylor, K ;

Whitehead, S ;

Woodward, JR ;

Barrell, BG .

NATURE, 2001, 409 (6823) :1007-1011

[6]

Doherty TM, 2002, J CLIN MICROBIOL, V40, P704, DOI 10.1128/JCM.40.2.704-706.2002

[7]

DOLIN PJ, 1994, B WORLD HEALTH ORGAN, V72, P213

[8] Purification of His-tagged proteins by immobilized chelate affinity chromatography: The benefits from the use of organic solvent [J].

Franken, KLMC ;

Hiemstra, HS ;

van Meijgaarden, KE ;

Subronto, Y ;

den Hartigh, J ;

Ottenhoff, THM ;

Drijfhout, JW .

PROTEIN EXPRESSION AND PURIFICATION, 2000, 18 (01) :95-99

[9] Identification and characterization of the ESAT-6 homologue of Mycobacterium leprae and T-cell cross-reactivity with Mycobacterium tuberculosis [J].

Geluk, A ;

van Meijgaarden, KE ;

Franken, KLMC ;

Subronto, YW ;

Wieles, B ;

Arend, SM ;

Sampaio, EP ;

de Boer, T ;

Faber, WR ;

Naafs, B ;

Ottenhoff, THM .

INFECTION AND IMMUNITY, 2002, 70 (05) :2544-2548

[10]

HASLOV K, 1995, INFECT IMMUN, V63, P804

← 1 2 →