Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

被引：34

作者：

Chen, S

Lu, D

Zhang, M

Che, J

Yin, Z

Zhang, S

Zhang, W

Bo, X

Ding, Y

Wang, S

机构：

[1] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

[2] Glodenweikai Med Biotechnol Co Ltd, Beijing 100850, Peoples R China

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2005年 / 24卷 / 08期

关键词：

D O I：

10.1007/s10096-005-1378-7

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.

引用

页码：549 / 553

页数：5

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