Characterization of clonogenic stromal cells isolated from human endometrium

被引:113
作者
Dimitrov, R. [2 ]
Timeva, T. [3 ]
Kyurkchlev, D. [4 ]
Stamenova, M. [2 ]
Shterev, A. [3 ]
Kostova, P. [5 ]
Zlatkov, V. [6 ]
Kehayov, I. [1 ]
Kyurkchiev, S. [1 ]
机构
[1] Bulgarian Acad Sci, Inst Biol & Immunol Reprod, Dept Mol Immunol, BU-1113 Sofia, Bulgaria
[2] Bulgarian Acad Sci, Inst Biol & Immunol Reprod, Dept Immunochem, BU-1113 Sofia, Bulgaria
[3] Ob Gy Hosp Dr Shterev, Sofia 1330, Bulgaria
[4] Univ Hosp Svl Rilski, Clin Immunol Lab, Sofia 1431, Bulgaria
[5] Natl Oncol Hosp, Ginecol Clin, Sofia 1756, Bulgaria
[6] Univ Og Gyn hosp Maichin Dom, Sofia 1437, Bulgaria
关键词
D O I
10.1530/REP-07-0428
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human endometrium is an object of extensive restructuring and remodeling during the female reproductive life and it is quite tempting to assume that these periodic changes happen with the participation of cells that should have the basic characteristics of multipotent cells. The aim of this study was to search for the presence of cells with plastic adherence, clonogenicity, and differentiation in human endometrium. To this end, human endometrial stromal cells were cultured in vitro for more than 15 passages. Flow cytometry analysis of the cultured cells showed that they were positive for CD29, CD73 and CD90, which are considered to be the markers of cells with mesenchymal origin. The cells were negative for the hematopoietic cell markers (CD45, CD34, CD14, CD3. CD19, CD16/56, and HLA-DR). Further, it was shown that the cultured cells had 15% clonogenic efficiency and could be induced to differentiate into adipogenic cells containing typical lipid-rich vacuoles. These results demonstrate that the human endometrium contains a low number of cells with the characteristics of endometrial stromal stem/progenitor cells, which seem to belong to the family of the mesenchymal stem cells. It can he speculated that these cells are engaged into the monthly restructuring and remodeling of human endometrium.
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收藏
页码:551 / 558
页数:8
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