Priming of human neutrophil superoxide generation by tumour necrosis factor-α is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation

被引：38

作者：

Condliffe, AM

Hawkins, PT

Stephens, LR

Haslett, C

Chilvers, ER

机构：

[1] Univ Edinburgh, Rayne Lab, Dept Med RIE, Resp Med Unit, Edinburgh EH8 9AG, Midlothian, Scotland

[2] Babraham Inst, Dept Signalling, Cambridge CB2 4AT, England

来源：

FEBS LETTERS | 1998年 / 439卷 / 1-2期

基金：

英国惠康基金;

关键词：

neutrophil; tumor necrosis factor; phosphoinositide; 3-hydroxykinase;

D O I：

10.1016/S0014-5793(98)01358-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human neutrophils, significant agonist-stimulated superoxide anion (O-2(-)) release is observed only after exposure to a priming agent such as TNF alpha. We have investigated the potential for TNF alpha to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P-3 and PtdIns(3,4,5)P-3 accumulation. TNP alpha pretreatment did not affect basal or stimulated Ins(1,4,5)P-3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P-3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O-2(-) generation implying a possible role for PtdIns(3,4,5)P-3 in signalling primed O-2(-) release. (C) 1998 Federation of European Biochemical Societies.

引用

页码：147 / 151

页数：5

共 32 条

[1] ACTIVATION OF NADPH OXIDASE INVOLVES THE DISSOCIATION OF P21(RAC) FROM ITS INHIBITORY GDP/GTP EXCHANGE PROTEIN (RHOGDI) FOLLOWED BY ITS TRANSLOCATION TO THE PLASMA-MEMBRANE
ABO, A
WEBB, MR
GROGAN, A
SEGAL, AW
[J]. BIOCHEMICAL JOURNAL, 1994, 298 : 585 - 591
[2] CYCLIC AMP-INCREASING AGENTS INTERFERE WITH CHEMOATTRACTANT INDUCED RESPIRATORY BURST IN NEUTROPHILS AS A RESULT OF THE INHIBITION OF PHOSPHATIDYLINOSITOL 3-KINASE RATHER THAN RECEPTOR-OPERATED CA2+ INFLUX
AHMED, MU
HAZEKI, K
HAZEKI, O
KATADA, T
UI, M
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) : 23816 - 23822
[3] LEUKOCYTE ADHESION DEFICIENCY - AN INHERITED DEFECT IN THE MAC-1, LFA-1, AND P150,95 GLYCOPROTEINS
ANDERSON, DC
SPRINGER, TA
[J]. ANNUAL REVIEW OF MEDICINE, 1987, 38 : 175 - 194
[4] WORTMANNIN IS A POTENT PHOSPHATIDYLINOSITOL 3-KINASE INHIBITOR - THE ROLE OF PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE IN NEUTROPHIL RESPONSES
ARCARO, A
WYMANN, MP
[J]. BIOCHEMICAL JOURNAL, 1993, 296 : 297 - 301
[5] BERKOW RL, 1987, J IMMUNOL, V139, P3783
[6] MASS MEASUREMENTS OF INOSITOL(1,4,5)TRISPHOSPHATE IN RAT CEREBRAL-CORTEX SLICES USING A RADIORECEPTOR ASSAY - EFFECTS OF NEUROTRANSMITTERS AND DEPOLARIZATION
CHALLISS, RAJ
BATTY, IH
NAHORSKI, SR
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (02) : 684 - 691
[7] PDGF-DEPENDENT AND INSULIN-DEPENDENT PP70(S6K) ACTIVATION MEDIATED BY PHOSPHATIDYLINOSITOL-3-OH KINASE
CHUNG, JK
GRAMMER, TC
LEMON, KP
KAZLAUSKAS, A
BLENIS, J
[J]. NATURE, 1994, 370 (6484) : 71 - 75
[8] COREY SJ, 1989, J BIOL CHEM, V264, P14165
[9] THE INHIBITION OF GLYCOGEN-SYNTHASE KINASE-3 BY INSULIN OR INSULIN-LIKE GROWTH-FACTOR-1 IN THE RAT SKELETAL-MUSCLE CELL-LINE-L6 IS BLOCKED BY WORTMANNIN, BUT NOT BY RAPAMYCIN - EVIDENCE THAT WORTMANNIN BLOCKS ACTIVATION OF THE MITOGEN-ACTIVATED PROTEIN-KINASE PATHWAY IN L6-CELLS BETWEEN RAS AND RAF
CROSS, DAE
ALESSI, DR
VANDENHEEDE, JR
MCDOWELL, HE
HUNDAL, HS
COHEN, P
[J]. BIOCHEMICAL JOURNAL, 1994, 303 : 21 - 26
[10] THE REQUIREMENT FOR PHOSPHOLIPASE-A(2) FOR ACTIVATION OF THE ASSEMBLED NADPH OXIDASE IN HUMAN NEUTROPHILS
DANA, R
MALECH, HL
LEVY, R
[J]. BIOCHEMICAL JOURNAL, 1994, 297 : 217 - 223

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