Numerous and highly developed tubular projections from plastids observed in Tobacco epidermal cells

被引：31

作者：

Arimura, S ^{[1
]}

Hirai, A ^{[1
]}

Tsutsumi, N ^{[1
]}

机构：

[1] Univ Tokyo, Plant Mol Genet Lab, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

PLANT SCIENCE | 2001年 / 160卷 / 03期

基金：

日本学术振兴会;

关键词：

plastid connection; stromule; plastid tubular projection; confocal laser scanning microscope; GFP; Nicotiana tabacum;

D O I：

10.1016/S0168-9452(00)00405-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tubular projections from plastids (stromules) were observed using a stroma-targeted green fluorescent protein (GFP) fusion protein and a confocal laser scanning microscope. Tn tobacco (Nicotiana tabacum) epidermal cells, stromules were observed at a high frequency. Some of them were long and connected plastids. Three days after particle bombardment, 80.6 +/- 6.99% of the transformed cells contained some plastids with more than one stromule, and 40.2 +/- 7.7% contained at least one pair of plastids connected by stromules. In a few cells, numerous and highly developed stromules covering the whole cell were observed. Stromules were also observed in epidermal cells in each of three other plant species that were tested: rice, dayflower (Commelina communis) and Arabidopsis thaliana. These findings demonstrated that stromules are common structure in plant epidermal cells. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：449 / 454

页数：6

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