Fasudil attenuates sympathetic nervous activity in the adrenal medulla of spontaneously hypertensive rats

被引：11

作者：

Kumai, Toshio ^{[1
]}

Takeba, Yuko ^{[1
]}

Matsumoto, Naoki ^{[1
]}

Nakaya, Sachiko ^{[1
]}

Tsuzuki, Yoshimitsu ^{[1
]}

Yanagida, Yohei ^{[1
]}

Hayashi, Mikito ^{[1
]}

Kobayashi, Shinichi ^{[1
]}

机构：

[1] St Marianna Univ, Sch Med, Dept Pharmacol, Miyamae Ku, Kawasaki, Kanagawa 2168511, Japan

来源：

LIFE SCIENCES | 2007年 / 81卷 / 15期

关键词：

Fasudil; Rho kinase; catecholamine; tyrosine hydroxylase; SHR; adrenal medulla;

D O I：

10.1016/j.lfs.2007.08.008

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We investigated the effects of fasudil, a Rho kinase inhibitor, on hypertension in spontaneously hypertensive rats and on the cateebolamine synthetic pathway. Ten-week-old male SHR and Wistar-Kyoto rats were administered fasudil (10 mg/kg/day s.c.) for 4 days. Systolic blood pressure was measured using the tail-cuff method. Catecholamme levels were measured with high-performance liquid chromatography-ECD methods. Tyrosine hydroxylase protein levels were measured in Western blot analysis. The tyrosine hydroxylase mRNA level was measured using real-time PCR methods. Fasudil significantly decreased systolic blood pressure in spontaneously hypertensive rats, but not in Wistar-Kyoto rats. Fasudil also significantly decreased catecholamine, tyrosine hydroxylase protein, and tyrosine hydroxylase mRNA levels in the adrenal medulla of spontaneously hypertensive rats. These results suggest that the depressor effects of fasudil on hypertension in spontaneously hypertensive rats may be related to inhibition of the catecholamine synthetic pathway. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：1193 / 1198

页数：6

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