Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications

被引:113
作者
Le Blanc, JCY
Hager, JW
Ilisiu, AMP
Hunter, C
Zhong, F
Chu, I
机构
[1] Appl Biosyst Inc, Concord, ON LAK 4V8, Canada
[2] MDS Sciex, Concord, ON, Canada
[3] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
关键词
linear ion trap; liquid chromatography; mass spectrometry; phosphopeptide; protein digest;
D O I
10.1002/pmic.200300415
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The unique scanning capabilities of a hybrid linear ion trap (Q TRAP) mass spectrometer are described with an emphasis on proteomics applications. The combination of the very selective triple quadrupole based tandem mass spectrometry (MS/MS) scans with the very sensitive ion trap product ion scans allows rapid identification of peptides at low concentrations derived from post-translationally modified proteins on chromatographic time scales. The Q TRAP instrument also offers the opportunity to conduct a variety of ion processing steps prior to performing a mass scan. For example, the enhancement of the multiple-charge ion contents of the ion trap can be performed resulting in a survey mass spectrum dominated by double- and triple-charge peptides. This facilitates the identification of relevant biological species in both separated and unseparated peptide mixtures for further MS/MS experiments.
引用
收藏
页码:859 / 869
页数:11
相关论文
共 9 条
[1]   Mass spectrometry in proteomics [J].
Aebersold, R ;
Goodlett, DR .
CHEMICAL REVIEWS, 2001, 101 (02) :269-295
[2]  
COVEY TR, Patent No. 5952653
[3]   A new linear ion trap mass spectrometer [J].
Hager, JW .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (06) :512-526
[4]  
HAGER JW, 2002, Patent No. 0175279
[5]   Analysis of proteins and proteomes by mass spectrometry [J].
Mann, M ;
Hendrickson, RC ;
Pandey, A .
ANNUAL REVIEW OF BIOCHEMISTRY, 2001, 70 :437-473
[6]   Proteomics: the move to mixtures [J].
Peng, JM ;
Gygi, SP .
JOURNAL OF MASS SPECTROMETRY, 2001, 36 (10) :1083-1091
[7]   Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode [J].
Steen, H ;
Kuster, B ;
Fernandez, M ;
Pandey, A ;
Mann, M .
ANALYTICAL CHEMISTRY, 2001, 73 (07) :1440-1448
[8]   Implementation and uses of automated de novo peptide sequencing by tandem mass spectrometry [J].
Taylor, JA ;
Johnson, RS .
ANALYTICAL CHEMISTRY, 2001, 73 (11) :2594-2604
[9]   Parent ion scans of unseparated peptide mixtures [J].
Wilm, M ;
Neubauer, G ;
Mann, M .
ANALYTICAL CHEMISTRY, 1996, 68 (03) :527-533