Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide propylene oxide random copolymer in aqueous solution

被引：22

作者：

Cunha, MT

Tjerneld, F

Cabral, JMS

Aires-Barros, MR ^{[1
]}

机构：

[1] Inst Super Tecn, Ctr Engn Biol & Quim, P-1000 Lisbon, Portugal

[2] Univ Lund, Dept Biochem, S-22100 Lund, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 711卷 / 1-2期

关键词：

aqueous two-phase systems; thermoseparation; electrolytes; surfactants; ethylene oxide-propylene oxide copolymer; Breox;

D O I：

10.1016/S0378-4347(98)00021-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The thermoseparation of aqueous solutions of Breox 50 A 1000, an ethylene oxide-propylene oxide 50:50 (w/w) random copolymer, was studied. The cloud-point diagram for Breox in water solution and the effects of electrolytes and surfactants on the cloud-point temperature (CPT) were determined. The Breox concentration in both phases after the thermoseparation was followed with a reversed-phase HPLC method. The effects of separation temperature and additives on phase composition were evaluated. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：53 / 60

页数：8

共 23 条

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Albertsson P., 1986, Partitioning of Cell Particles and Macromolecules, DOI DOI 10.1002/CBF.290050311

[2]

APPLICATION OF TEMPERATURE-INDUCED PHASE PARTITIONING AT AMBIENT-TEMPERATURE FOR ENZYME-PURIFICATION [J].