Bacterial Community Diversity in the Brazilian Atlantic Forest Soils

被引：73

作者：

Bruce, Thiago ^{[2
]}

Martinez, Ivana B. ^{[2
,3
]}

Maia Neto, Oswaldo ^{[2
]}

Vicente, Ana Carolina P. ^{[4
]}

Kruger, Ricardo H. ^{[5
]}

Thompson, Fabiano L. ^{[1
,2
]}

机构：

[1] Univ Fed Rio de Janeiro, Microbiol Lab, Ctr Ciencias Saude, Ilha Fundao, BR-21941902 Rio de Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Dept Genet, Inst Biol, BR-21941902 Rio de Janeiro, Brazil

[3] UFF, Dept Microbiol & Parasitol, Program Appl Microbiol & Parasitol MIP, Rio De Janeiro, Brazil

[4] IOC FIOCRUZ, Dept Genet, Rio De Janeiro, Brazil

[5] Univ Brasilia, UNB, Dept Enzymol, BR-70790160 Brasilia, DF, Brazil

来源：

MICROBIAL ECOLOGY | 2010年 / 60卷 / 04期

关键词：

PHYLOGENETIC DIVERSITY; PROKARYOTIC DIVERSITY; MICROBIAL COMMUNITIES; CELLULOSE; ANTHROSOLS; SEQUENCES; DYNAMICS; ADJACENT; PROGRAM;

D O I：

10.1007/s00248-010-9750-2

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071301 [植物生态学];

摘要：

The aim of this study was to characterize the bacterial community diversity of the Brazilian Atlantic forest soil by means of both cultivation and 16S rRNA clone libraries. A collection of 86 representative isolates, obtained from six samples of Atlantic forest soils from the National Park of Serra dos Orgaos (PARNASO), belonged to the genera Arthrobacter, Bacillus, Burkholderia, Leifsonia, Paenibacillus, Pseudomonas, Ralstonia, Serratia, and Streptomyces according to the 16S rRNA sequences. Representative isolates from the different genera degraded cellulose and lignin. The culture-independent analysis based on 894 partial 16S rRNA gene sequences revealed that the most frequently retrieved groups belonged to the phyla Acidobacteria (29-54%), Proteobacteria (16-38%), and Verrucomicrobia (0.6-14%). The majority of the sequences (82.6%) were unidentified singletons and doubletons, indicating a high diversity of rare unique sequences. Chaol estimator disclosed a high number of phyla (41-152) and species (263-446). This is the first survey on the Atlantic Forest soils using a combination of cultivation and culture-independent approaches. We conclude that the Brazilian Atlantic Forest soil represents a vast source of novel bacteria.

引用

页码：840 / 849

页数：10

共 43 条

[1]

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

[Anonymous], 2005, PHYLIP (phylogeny inference package) version 3.6

[3]

The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy [J].

Cole, JR ;

Chai, B ;

Marsh, TL ;

Farris, RJ ;

Wang, Q ;

Kulam, SA ;

Chandra, S ;

McGarrell, DM ;

Schmidt, TM ;

Garrity, GM ;

Tiedje, JM .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :442-443

[4]

Microbial diversity in alpine tundra wet meadow soil: novel Chloroflexi from a cold, water-saturated environment [J].

Costello, Elizabeth K. ;

Schmidt, Steven K. .

ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (08) :1471-1486

[5]

Despite strong seasonal responses, soil microbial consortia are more resilient to long-term changes in rainfall than overlying grassland [J].

Cruz-Martinez, Karelyn ;

Suttle, K. Blake ;

Brodie, Eoin L. ;

Power, Mary E. ;

Andersen, Gary L. ;

Banfield, Jillian F. .

ISME JOURNAL, 2009, 3 (06) :738-744

[6]

Estimating prokaryotic diversity and its limits [J].

Curtis, TP ;

Sloan, WT ;

Scannell, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (16) :10494-10499

[7]

Phylogenetic analysis of partial bacterial 16S rDNA sequences of tropical grass pasture soil under Acacia tortilis subsp. raddiana in Senegal [J].

Diallo, MD ;

Martens, M ;

Vloemans, N ;

Cousin, S ;

Vandekerckhove, TTM ;

Neyra, M ;

de Lajudie, P ;

Willems, A ;

Gillis, M ;

Vyverman, W ;

Van der Gucht, K .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2004, 27 (02) :238-252

[8]

CELLULOSE AND LIGNIN DEGRADATION IN FOREST SOILS - RESPONSE TO MOISTURE, TEMPERATURE, AND ACIDITY [J].

DONNELLY, PK ;

ENTRY, JA ;

CRAWFORD, DL ;

CROMACK, K .

MICROBIAL ECOLOGY, 1990, 20 (03) :289-295

[9]

Novelty and uniqueness patterns of rare members of the soil biosphere [J].

Elshahed, Mostafa S. ;

Youssef, Noha H. ;

Spain, Anne M. ;

Sheik, Cody ;

Najar, Fares Z. ;

Sukharnikov, Leonid O. ;

Roe, Bruce A. ;

Davis, James P. ;

Schloss, Patrick D. ;

Bailey, Vanessa L. ;

Krumholz, Lee R. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (17) :5422-5428

[10]

List of bacterial names with standing in nomenclature: A folder available on the Internet [J].

Euzeby, JP .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (02) :590-592

← 1 2 3 4 5 →