Aberrant cyclooxygenase isozyme expression in human intrahepatic cholangiocarcinoma

被引:66
作者
Chariyalertsak, S
Sirikulchayanonta, V
Mayer, D
Kopp-Schneider, A
Fürstenberger, G
Marks, F
Müller-Decker, K
机构
[1] Deutsch Krebsforschungszentrum, Div Biochem Tissue Specific Regulat, D-69120 Heidelberg, Germany
[2] Natl Canc Inst, Div Res, Bangkok, Thailand
[3] Mahidol Univ, Ramathibodi Hosp, Dept Pathol, Surg Pathol Sect, Bangkok 10400, Thailand
[4] Deutsch Krebsforschungszentrum, Div Cell Pathol, D-6900 Heidelberg, Germany
[5] Deutsch Krebsforschungszentrum, Cent Unit Biostat, D-6900 Heidelberg, Germany
关键词
cyclooxygenase; immunohistochemistry; cholangiocarcinoma; bile duct; hepatocyte; Kupffer cell;
D O I
10.1136/gut.48.1.80
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Methods-Cellular localisation of the cyclooxygenase (COX) isozymes COX-1 and COX-2 was analysed in 24 cholangiocarcinomas, including 17 matched tissues originating from non-tumorous liver tissue adjacent to tumours and seven biopsies of normal human liver, by immunohistochemistry using isozyme selective antibodies. Results-In normal liver, constitutive expression of COX-2 protein was a characteristic feature of hepatocytes whereas no COX-2 immunosignal was detectable in normal bile duct epithelium, Kupffer, and endothelial cells. In cholangiocarcinoma cells, COX-2 protein was strongly expressed at high frequency. The intensity, percentage of positive cells, and pattern of COX-2 expression were found to be independent of the stage of tumour differentiation. In hepatocytes of matched nontumorous tissue, COX-2 expression was unaltered. In contrast, strong COX-1 expression was frequently localised to Kupffer cells, endothelial cells, and occasionally to hepatocytes, but not to bile duct epithelial cells. In approximately half of moderately and poorly differentiated but not well differentiated cholangiocarcinomas, weak to moderate COX-1 staining was found in tumour cells while COX-1 expression in Kupffer cells was much more pronounced. Conclusion-Aberrant COX-2 expression occurs during the early stage while COX-1 over expression seems to be related to later stages of cholangiocarcinogenesis.
引用
收藏
页码:80 / 86
页数:7
相关论文
共 42 条
[1]   Chemoprevention of spontaneous intestinal adenomas in the adenomatous polyposis coli Min mouse model with aspirin [J].
Barnes, CJ ;
Lee, M .
GASTROENTEROLOGY, 1998, 114 (05) :873-877
[2]  
Boolbol SK, 1996, CANCER RES, V56, P2556
[3]   COX-2 expression is induced by UVB exposure in human skin: Implications for the development of skin cancer [J].
Buckman, SY ;
Gresham, A ;
Hale, P ;
Hruza, G ;
Anast, J ;
Masferrer, J ;
Pentland, AP .
CARCINOGENESIS, 1998, 19 (05) :723-729
[4]  
Chan G, 1999, CANCER RES, V59, P991
[5]  
CHINTHALAPALLY VR, 1995, CANCER RES, V55, P1464
[6]   Cyclooxygenase in biology and disease [J].
Dubois, RN ;
Abramson, SB ;
Crofford, L ;
Gupta, RA ;
Simon, LS ;
Van De Putte, LBA ;
Lipsky, PE .
FASEB JOURNAL, 1998, 12 (12) :1063-1073
[7]   UP-REGULATION OF CYCLOOXYGENASE-2 GENE-EXPRESSION IN HUMAN COLORECTAL ADENOMAS AND ADENOCARCINOMAS [J].
EBERHART, CE ;
COFFEY, RJ ;
RADHIKA, A ;
GIARDIELLO, FM ;
FERRENBACH, S ;
DUBOIS, RN .
GASTROENTEROLOGY, 1994, 107 (04) :1183-1188
[8]   EICOSANOIDS AND MULTISTAGE CARCINOGENESIS IN NMRI MOUSE SKIN - ROLE OF PROSTAGLANDIN-E AND PROSTAGLANDIN-F IN CONVERSION (1ST STAGE OF TUMOR PROMOTION) AND PROMOTION (2ND STAGE OF TUMOR PROMOTION) [J].
FURSTENBERGER, G ;
GROSS, M ;
MARKS, F .
CARCINOGENESIS, 1989, 10 (01) :91-96
[9]   TREATMENT OF COLONIC AND RECTAL ADENOMAS WITH SULINDAC IN FAMILIAL ADENOMATOUS POLYPOSIS [J].
GIARDIELLO, FM ;
HAMILTON, SR ;
KRUSH, AJ ;
PIANTADOSI, S ;
HYLIND, LM ;
CELANO, P ;
BOOKER, SV ;
ROBINSON, CR ;
OFFERHAUS, GJA .
NEW ENGLAND JOURNAL OF MEDICINE, 1993, 328 (18) :1313-1316
[10]  
Hao XP, 1999, J PATHOL, V187, P295