Molecular detection of Yersinia pestis isolates of Indian origin by using Pla specific monoclonal antibodies

被引：13

作者：

Mahesh, S ^{[1
]}

Shukla, J ^{[1
]}

Tuteja, U ^{[1
]}

Batra, H ^{[1
]}

机构：

[1] Def Res & Dev Estab, Div Microbiol, Gwalior 474002, India

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2005年 / 28卷 / 02期

关键词：

plasminogen activator; recombinant protein; monoclonal antibody; Indian isolates of Yersinia pestis; DNA probe;

D O I：

10.1016/j.cimid.2004.08.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Monoclonal antibodies (MAbs) were generated against the recombinant plasminogen activator (Pla) protein of Yersinia pestis. These MAbs detected Pla in all the 18 isolates of Y pestis obtained from the sputum of pneumonic plague patients and from the liver and spleen of rodents from plague-affected areas of India during 1994-1995 as well as in seven of the eight isolates obtained from rodents in the surveillance regions of Hosur and Palmner in India during 1998 by simple dot-ELISA. In immunoblotting, the MAbs reacted with the Pla antigen only in Y. pestis isolates at 37 and 35 kDa region. These monoclonal antibodies, being strictly specific, can be used for detecting Y. pestis isolates that are Fraction I antigen-negative. Also, the radiolabelled pla fragment hybridized specifically to the representative DNA samples of Y. pestis isolates. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：131 / 144

页数：14

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