Membrane protein crystallization in amphiphile phases: practical and theoretical considerations

被引：23

作者：

Nollert, P ^{[1
]}

机构：

[1] DeCODE BioStruct, Bainbridge Isl, WA 98110 USA

来源：

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2005年 / 88卷 / 03期

关键词：

membrane protein crystallization; amphiphile; crystal packing; lipid; detergent;

D O I：

10.1016/j.pbiomolbio.2004.07.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Integral membrane proteins are amphiphilic molecules. In order to enable chromatographic purification and crystallization, a complementary amphiphilic microenvironment must be created and maintained. Various types of amphiphilic phases have been employed in crystallizations and intricate amphiphilic microenvironmental. structures have resulted from these and are found inside membrane protein crystals. In this review the process of crystallization is put into the context of amphiphile phase transitions. Finally, practical factors are considered and a pragmatic way is suggested to pursue membrane protein crystallization trials. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：339 / 357

页数：19

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