Effects of nitric oxide synthase inhibitors on the outcome of in vitro fertilization in the mouse

被引:28
作者
Herrero, MB
Viggiano, JM
Martinez, SP
deGimeno, MF
机构
[1] Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Buenos Aires
关键词
D O I
10.1071/RD9960301
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The effect of three nitric oxide (NO) synthase inhibitors, L-N-G-nitro-arginine (NO(2)Arg), N-G-Nitro-L-arginine methyl ester (L-NAME) and aminoguanidine, on in vitro fertilization in the mouse was examined. Mouse epididymal spermatozoa were capacitated in a medium with or without NO synthase inhibitors. Oocytes were inseminated and the percentage of oocytes with two pronuclei was scored after an 8-h incubation. NO(2)Arg and L-NAME, but not aminoguanidine, inhibited fertilization. L-NAME inhibited fertilization in a dose-dependent manner, and its effects were stereospecific. The inhibitory effect was neutralized by L-arginine but not by D-arginine. Moreover, D-NAME did not inhibit fertilization. The results suggest that NO synthase activity (presumably of the constitutive type) is necessary for spermatozoa to display their full fertilizing ability.
引用
收藏
页码:301 / 304
页数:4
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