Intrinsically aged epidermis displays diminished UVB-induced alterations in barrier function associated with decreased proliferation

被引:53
作者
Haratake, A
Uchida, Y
Mimura, K
Elias, PM
Holleran, WM
机构
[1] KANEBO LTD, COSMET LAB, ODAWARA, KANAGAWA, JAPAN
[2] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT DERMATOL, SAN FRANCISCO, CA 94143 USA
[3] VET ADM MED CTR, DERMATOL SERV, SAN FRANCISCO, CA 94121 USA
关键词
epidermal permeability barrier; aging; DNA synthesis; epidermal hyperplasia;
D O I
10.1111/1523-1747.ep12286474
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Ultraviolet (UV) irradiation of the skin induces a variety of responses in the epidermis, including sunburn cell formation, epidermal hyperplasia, and a dose-dependent permeability barrier abnormality, an effect that appears to be dependent upon both UVB-induced hyperplasia and T-cell activation, Since intrinsically aged epidermis displays decreased epidermal turnover, diminished inflammatory response to various stimuli, including UVR, and impaired immune function, we investigated the effects of UVB on both epidermal barrier function and proliferation in hairless mice of increasing chronologic age (27, 61, and 90 wk), After a single UVB exposure (0.15 J/cm(2) approximate to 7.5 MED), a barrier abnormality developed (i.e., increased transepidermal water loss; TEWL), after a delay of greater than or equal to 48 h, regardless of age, In young mice (27 wk old), TEWL levels peaked at 72-96 h (9.9-fold over untreated controls), whereas increased epidermal [H-3]thymidine incorporation preceded the peak TEWL increase (i.e., approximate to 570% increase over controls at 48 h), In contrast, the UVB-induced increased in both TEWL and DNA synthesis were significantly diminished, with decreased epidermal hyperplasia evident, in intrinsically aged versus young mouse epidermis, Baseline epidermal thickness decreased with animal age (i.e., 16.8 +/- 3.1 vs. 27.9 +/- 0.7 mu m for 90- vs. 27-wk-old animals, respectively; p < 0.02), suggesting that the diminished barrier response with aging reflects an attenuation of events subsequent to initial UVB exposure, rather than an increase in the UV dose delivered, These results demonstrate that (i) murine epidermis becomes less sensitive to UVB-induced barrier alterations with age and (ii) decreased DNA synthesis after UVB correlates with the age-related decrease in barrier dysfunction.
引用
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页码:319 / 323
页数:5
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