Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

被引：64

作者：

Matsunaga, T

Sato, R

Kamiya, S

Tanaka, T

Takeyama, H

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

[2] TDK Akita Lab Corp, Akita 0180493, Japan

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 1999年 / 194卷 / 1-3期

关键词：

bacterial magnetic particles (BMPs); ProteinA BMP complex; chemiluminescence enzyme immunoassay; magA gene;

D O I：

10.1016/S0304-8853(98)00575-7

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-10(3) ng/ml. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：126 / 131

页数：6

共 19 条

[1] ULTRASTRUCTURE OF A MAGNETOTACTIC SPIRILLUM [J].