Enterohemorrhagic Escherichia coli exploits EspA filaments for attachment to salad leaves

被引：79

作者：

Shaw, Robert K. ^{[2
]}

Berger, Cedric N. ^{[1
]}

Feys, Bart ^{[3
]}

Knutton, Stuart ^{[2
]}

Pallen, Mark J. ^{[2
]}

Frankel, Gad ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Div Cell & Mol Biol, London SW7 2AZ, England

[2] Univ Birmingham, Sch Med, Div Immun & Infect, Birmingham B15 2TT, W Midlands, England

[3] Univ London Imperial Coll Sci Technol & Med, Div Cell & Biol, London SW7 2AZ, England

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 09期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1128/AEM.02704-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enterohemorrhagic Escherichia coli (EHEC) strains are important food-borne pathogens that use a filamentous type III secretion system (fT3SS) for colonization of the gut epithelium. In this study we have shown that EHEC O157 and 026 strains use the fT3SS apparatus for attachment to leaves. Leaf attachment was independent of effector protein translocation.

引用

页码：2908 / 2914

页数：7

共 30 条

[1] Enterohaemorrhagic Escherichia coli:: emerging issues on virulence and modes of transmission [J].

Caprioli, A ;

Morabito, S ;

Brugère, H ;

Oswald, E .

VETERINARY RESEARCH, 2005, 36 (03) :289-311

[2] Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells:: role of bundle-forming pili (BFP), EspA filaments and intimin [J].

Cleary, J ;

Lai, LC ;

Shaw, RK ;

Straatman-Iwanowska, A ;

Donnenberg, MS ;

Frankel, G ;

Knutton, S .

MICROBIOLOGY-SGM, 2004, 150 :527-538

[3] Rapid modulation of electrolyte transport in Caco-2 cell monolayers by enteropathogenic Escherichia coli (EPEC) infection [J].

Collington, GK ;

Booth, IW ;

Knutton, S .

GUT, 1998, 42 (02) :200-207

[4] The type III secretion injectisome [J].

Cornelis, Guy R. .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (11) :811-825

[5] Polarity of enteropathogenic Escherichia coli EspA filament assembly and protein secretion [J].

Crepin, VF ;

Shaw, R ;

Abe, CM ;

Knutton, S ;

Frankel, G .

JOURNAL OF BACTERIOLOGY, 2005, 187 (08) :2881-2889

[6] One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].

Datsenko, KA ;

Wanner, BL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645

[7] Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157:H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation [J].

Dziva, Francis ;

Vlisidou, Isabella ;

Crepin, Valerie F. ;

Wallis, Timothy S. ;

Frankel, Gad ;

Stevens, Mark P. .

VETERINARY MICROBIOLOGY, 2007, 123 (1-3) :254-261

[8] Initial binding of Shiga toxin-producing Escherichia coli to host cells and subsequent induction of actin rearrangements depend on filamentous EspA-containing surface appendages [J].

Ebel, F ;

Podzadel, T ;

Rohde, M ;

Kresse, AU ;

Krämer, S ;

Deibel, C ;

Guzmán, CA ;

Chakraborty, T .

MOLECULAR MICROBIOLOGY, 1998, 30 (01) :147-161

[9] Protein delivery into eukaryotic cells by type III secretion machines [J].

Galan, Jorge E. ;

Wolf-Watz, Hans .

NATURE, 2006, 444 (7119) :567-573

[10] Escherichia coli O157:H7 in beef cattle presented for slaughter in the US:: Higher prevalence rates than previously estimated [J].

Gansheroff, LJ ;

O'Brien, AD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :2959-2961

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