Quantitative NMR for bioanalysis and metabolomics

被引：138

作者：

Barding, Gregory A., Jr. ^{[1
]}

Salditos, Ryan ^{[1
]}

Larive, Cynthia K. ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2012年 / 404卷 / 04期

基金：

美国国家科学基金会;

关键词：

qNMR; Bioanalysis; Isotope enrichment; Metabonomics; Metabolomics; Natural products; Biofluids; Tissues; HR-MAS; NUCLEAR-MAGNETIC-RESONANCE; TISSUE-TARGETED METABONOMICS; RED-BLOOD-CELLS; H-1-NMR SPECTROSCOPY; BIOMARKER IDENTIFICATION; WATER SUPPRESSION; QUALITY-CONTROL; DATA SETS; HR-MAS; H-1;

D O I：

10.1007/s00216-012-6188-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements on a wide variety of samples. In particular, qNMR has emerged as an important method for metabolomics studies where it is used for interrogation of large sets of biological samples and the resulting spectra are treated with multivariate statistical analysis methods. In this review, recent developments in instrumentation and pulse sequences will be discussed as well as the practical considerations necessary for acquisition of quantitative NMR experiments with an emphasis on their use for bioanalysis. Recent examples of the application of qNMR for metabolomics/metabonomics studies, the characterization of biologicals such as heparin, antibodies, and vaccines, and the analysis of botanical natural products will be presented and the future directions of qNMR discussed.

引用

页码：1165 / 1179

页数：15

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