The protein inhibitor of neuronal nitric oxide synthase (PIN):: characterization of its action on pure nitric oxide synthases

被引：47

作者：

Hemmens, B ^{[1
]}

Woschitz, S ^{[1
]}

Pitters, E ^{[1
]}

Klösch, B ^{[1
]}

Völker, C ^{[1
]}

Schmidt, K ^{[1
]}

Mayer, B ^{[1
]}

机构：

[1] Karl Franzens Univ Graz, Inst Pharmakol & Toxikol, A-8010 Graz, Austria

来源：

FEBS LETTERS | 1998年 / 430卷 / 03期

关键词：

nitric oxide; nitric oxide synthase; dimerization; isoenzyme; inhibitor protein;

D O I：

10.1016/S0014-5793(98)00704-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuronal NO synthase (nNOS) was discovered recently to interact specifically with the protein PIN (protein inhibitor of nNOS) [Jaffrey S.R. and Snyder S.H. (1996) Science 274, 774-777]. We have studied the effects on pure NOS enzymes of the same GST-tagged PIN used in the original paper, Unexpectedly, all NOS isoenzymes were inhibited. The IC50 for nNOS was 18 +/- 6 mu M GST-PIN with 63 nM nNOS after 30 min at 37 degrees C, Uncoupled NADPH oxidation was inhibited similarly, whereas cytochrome c reductase activity, the K-M for L-arginine, and dimerization were unaffected. We reconsider the physiological role of PIN in the light of these results. (C) 1998 Federation of European Biochemical Societies.

引用

页码：397 / 400

页数：4

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