The number of direct repeats in hagA is variable among Porphyromonas gingivalis strains

The coding sequence for the surface protein hemagglutinin A (HagA) of Porphyromonas gingivalis 381 has previously been shown to contain four direct 1.35-kb repeats, designated rep,. This study was performed to determine if the number of rep(HA) units in hagA is consistently 4 or if allelic polymorphism exists among strains and/or upon multiple passage of P, gingivalis, To this end, primers which were homologous to the regions directly 5' and 3' of the repeat domain in hagA were synthesized. PCR conditions which allowed amplification of the 8.4-kb repeat region between the primers in P, gingivalis 381 were established. Genomic DNA templates from 13 other P, gingivalis strains and 9 fresh clinical isolates from patients were analyzed under the same conditions as used above. Analysis of these PCR products demonstrated that the strains tested had different numbers (two to four) of rep, units in the respective hagA genes, The PCR products of 8.4, 7.0, and 5.7 kb represent four, three, and two repeats, respectively, One strain from each group (381, four repeats; W83, three repeats; and AJW4, two repeats) was also tested to determine if the number of repeats remained invariant upon passaging onto solid medium, No variability in the number of repeats in hagA within a strain was detected after 18 passages. P, gingivalis 381 was chosen for further testing in a mouse abscess model to determine if conditions of in vivo growth would select for deletions or duplications of the repeated sequences. Five days after infection, no change in the number of repeats was detected in cells recovered from either nonimmunized or preimmunized mice. This data indicates an interstrain variability of the number of repeat units and hence a size variability of the HagA protein of P, gingivalis, but unlike some surface antigens of other pathogenic species, the number of repeats remains relatively stable given the conditions of growth tested here.