Inhibition of human immunodeficiency virus type 1 virion entry by dominant-negative Hck

被引：20

作者：

Tokunaga, K

Kiyokawa, E

Nakaya, M

Otsuka, N

Kojima, A

Kurata, T

Matsuda, M

机构：

[1] Int Med Ctr Japan, Res Inst, Dept Pathol, Shinjuku Ku, Tokyo 1628655, Japan

[2] Natl Inst Infect Dis, Dept Pathol, Shinjuku Ku, Tokyo 1628640, Japan

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 07期

关键词：

D O I：

10.1128/JVI.72.7.6257-6259.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To study the role of Src family tyrosine kinases in infection with human immunodeficiency virus type 1 (HIV-1), we constructed an Hck mutant, HckN, that hinders signaling from wild-type Hck. HIV-1 produced in HckN-expressing cells was significantly less infectious to HeLa-CD4-LTR-beta-gal (MAGI) cells than HIV-1 produced in mock-transfected cells. The inhibitory effect of HckN was compensated for by the expression of vesicular stomatitis virus G protein. Finally, we found that the HIV-1 produced in the HckN-expressing cells entered into the cells less efficiently than did the control HIV-1. These results suggest that the Src family tyrosine kinases regulate entry of HIV-1 into target cells.

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页码：6257 / 6259

页数：3

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