Morin applied in speciation of aluminium in natural waters and biological samples by reversed-phase high-performance liquid chromatography with fluorescence detection

被引：40

作者：

Lian, HZ

Kang, YF

Bi, SP

Yasin, A

Shao, DL

Chen, YJ

Dai, LM

Tian, LC

机构：

[1] Nanjing Univ, State Key Lab Coordinat Chem, Dept Chem, Nanjing 210093, Peoples R China

[2] Nanjing Univ, Ctr Mat Anal, Nanjing 210093, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2003年 / 376卷 / 04期

关键词：

aluminium; speciation; HPLC; morin;

D O I：

10.1007/s00216-003-1936-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic method with fluorescence detection for the determination of labile monomeric aluminium has been developed through pre-column complexation using morin as the analytical reagent. The highly fluorescent aluminium-morin complex (excitation wavelength 418 nm, emission wavelength 490 nm) was separated on a Spherisorb ODS 2 column with an eluent consisting of 30% methanol and 70% water (pH 1.0 with perchloric acid). The most remarkable point of this protocol was that only the most toxic aluminium species, that is, free aqua-aluminium ion and its monomeric hydroxo complex ions, selectively respond among various aluminium complexes. This strategy has been successfully applied to direct fractionation of the toxic aluminium in natural waters and biological samples without any pretreatment.

引用

页码：542 / 548

页数：7

共 38 条

[1] SPECTROPHOTOMETRIC DETERMINATION OF ALUMINUM BY MORIN [J].