Sample purification for the analysis of caffeine in tobacco by gas chromatography mass spectrometry

被引:9
作者
Song, SQ [1 ]
Ashley, DL [1 ]
机构
[1] Ctr Dis Control & Prevent, Div Environm Hlth Lab Sci, Natl Ctr Environm Hlth, Atlanta, GA 30341 USA
关键词
tobacco; caffeine; alkaloids;
D O I
10.1016/S0021-9673(98)00384-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A commonly used additive to tobacco products is cocoa. A sensitive and selective method was developed to measure caffeine, a marker for cocoa, in tobacco by using gas chromatography-mass spectrometry (GC-MS). Tobacco components usually produce high background signals in GC-MS analysis. Therefore, a series of extraction steps were designed to effectively purify the tobacco extracts. The analytical recovery of caffeine was 100% when [trimethyl-C-13(3)]caffeine was used as an isotope-dilution reference. A linear calibration curve was generated with caffeine concentration ranging from 0.01 to 20 mu g/ml. The detection limit of caffeine was 0.02 mu g/ml in the final solution. This method was applied to several commercial tobacco products, of which the corresponding caffeine levels varied from below the detection limit to 125 mu g/g. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:171 / 180
页数:10
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