Skeletal Muscle Lipase Content and Activity in Obesity and Type 2 Diabetes

被引:24
作者
Jocken, Johan W. E. [1 ]
Moro, Cedric [3 ]
Goossens, Gijs H. [1 ]
Hansen, Dominique [4 ,5 ]
Mairal, Aline [3 ]
Hesselink, Matthijs K. C. [2 ]
Langin, Dominique [3 ]
van Loon, Luc J. C. [2 ]
Blaak, Ellen E. [1 ]
机构
[1] Maastricht Univ, Med Ctr, Maastricht Sch Nutr Toxicol & Metab, Nutr & Toxicol Res Inst,Dept Human Biol, NL-6200 MD Maastricht, Netherlands
[2] Maastricht Univ, Med Ctr, Maastricht Sch Nutr Toxicol & Metab, Nutr & Toxicol Res Inst,Dept Human Movement Sci, NL-6200 MD Maastricht, Netherlands
[3] Inst Federatif Rech 31 Inst Louis Bugnard, Natl Inst Hlth & Med Res, Obes Res Ctr, Unit 858 I2MR Team 4, F-31432 Toulouse, France
[4] Prov Hogesch Limburg Univ Coll, Dept Healthcare, Jessa Hosp, B-3500 Hasselt, Belgium
[5] Prov Hogesch Limburg Univ Coll, Dept Healthcare, Rehabil & Healthcare Res Ctr, B-3500 Hasselt, Belgium
关键词
HORMONE-SENSITIVE LIPASE; INDUCED INSULIN-RESISTANCE; ADIPOSE TRIGLYCERIDE LIPASE; CERAMIDE CONTENT; TISSUE; LIPOLYSIS; HUMANS;
D O I
10.1210/jc.2010-0776
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Context: The obese insulin-resistant state is characterized by elevated lipid storage in skeletal muscle tissue. Objective: We tested whether differences in muscle triacylglycerol (TAG) and diacylglycerol (DAG) lipase content and activity are associated with incomplete in vivo lipolysis and lipid accumulation. Design and Patients: Two case-control studies were conducted on skeletal muscle biopsies from lean (n = 13) and obese (n = 10) men (study 1) and from 11 nonobese type 2 diabetic (T2D), obese T2D, and healthy normoglycemic men (study 2). Main Outcome Measures: Skeletal muscle lipase protein content and activity and muscle lipid content (TAG and DAG) were determined. Results: Skeletal muscle hormone-sensitive lipase protein content was lower (0.39 +/- 0.07 vs. 1.00 +/- 0.19 arbitrary units; P = 0.004) and adipose triglyceride lipase protein content was higher in obese men compared with lean controls (2.17 +/- 0.40 vs. 0.42 +/- 0.23 arbitrary units; P = 0.008). This apparent difference in lipase content was accompanied by a 60% lower ratio of DAG to TAG hydrolase activity in the obese men (11.4 +/- 2.3 vs. 26.5 +/- 7.3 nmol/h.mg; P = 0.045), implying incomplete lipolysis. Lower hormone-sensitive lipase and higher adipose triglyceride lipase content was confined to obesity per se, because it was observed solely in obese T2D men but not in healthy normoglycemic controls and nonobese T2D men. Muscle total DAG content was not higher in obese men but was even lower (6.2 +/- 0.7 vs. 9.4 +/- 0.9 mu mol/mg dry weight; P = 0.017). TAG content did not differ between groups (84.7 +/- 18.9 vs. 70.4 +/- 12.4 mu mol/mg dry weight; P = 0.543). Conclusions: Our data do not support an important role of total muscle DAG content in the development of insulin resistance in obese men. (J Clin Endocrinol Metab 95: 5449-5453, 2010)
引用
收藏
页码:5449 / 5453
页数:5
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