Geologically ancient DNA: fact or artefact?

被引:103
作者
Hebsgaard, MB
Phillips, MJ
Willerslev, E
机构
[1] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
[2] Univ Copenhagen, Niels Bohr Inst, Ctr Evolutionary Genet, DK-2100 Copenhagen, Denmark
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/j.tim.2005.03.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Studies continue to report ancient DNA sequences and viable microbial cells that are many millions of years old. In this paper we evaluate some of the most extravagant claims of geologically ancient DNA. We conclude that although exciting, the reports suffer from inadequate experimental setup and insufficient authentication of results. Consequently, it remains doubtful whether amplifiable DNA sequences and viable bacteria can survive over geological timescales. To enhance the credibility of future studies and assist in discarding false-positive results, we propose a rigorous set of authentication criteria for work with geologically ancient DNA.
引用
收藏
页码:212 / 220
页数:9
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