Evaluation of immunochromatography and commercial enzyme-linked immunosorbent assay for rapid detection of norovirus antigen in stool samples

被引:32
作者
Khamrin, Pattara [1 ]
Nguyen, Tuan Anh [1 ]
Phan, Tung Gia [1 ]
Satou, Kenji [2 ]
Masuoka, Yuichi [2 ]
Okitsu, Shoko [1 ]
Maneekarn, Niwat [3 ]
Nishio, Osamu [1 ]
Ushijima, Hiroshi [1 ]
机构
[1] Univ Tokyo, Grad Sch Med, Inst Int Hlth, Dept Dev Med Sci, Tokyo, Japan
[2] Immuno Probe Co Ltd, Ranzan, Saitama, Japan
[3] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai, Thailand
关键词
norovirus; immunochromatography; denka ELISA; RT-PCR; gastroenteritis;
D O I
10.1016/j.jviromet.2007.09.007
中图分类号
Q5 [生物化学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
The efficiency of immunochromatography and commercial enzyme-linked immunosorbent assay (ELISA) kit (Denka Seiken Co. Ltd., Tokyo, Japan) were evaluated for rapid detection of norovirus (NoV) from stool specimens. A total of 503 stool specimens collected from infants and young children who suffered from acute gastroenteritis were tested for NoV by the NoV-immunochromatography kit, Denka ELISA kit, and by a monoplex RT-PCR method. The NoV-immunochromatography revealed 78.9% sensitivity, 96.4% specificity, and 92.4% efficiency with the monoplex RT-PCR method. The Denka ELISA kit had a sensitivity of 90.4%, specificity of 96.4%, and an efficiency level of 95%. The findings indicate that the newly developed NoV-immunochromatography kit provides the specificity equal to that of the Denka ELISA kit, even through the sensitivity of detection was lower. However, the advantage of the NoV-immunochromatography kit is less time consuming and simpler. The data show that both the Denka ELISA and the NoV-immunochromatography kits may be used as an alternative method for screening of NoV in stool samples. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:360 / 363
页数:4
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