Isogloboside biosynthesis in metastatic R3230AC cells results from a decreased GM3 synthase activity

被引:5
作者
Dumonceaux, T
Carlsen, SA
机构
[1] Univ Saskatchewan, Coll Med, Dept Microbiol, Saskatoon, SK S7N 4H4, Canada
[2] Saskatchewan Canc Agcy, Canc Res Unit, Saskatoon, SK S7N 4H4, Canada
关键词
isogloboside; metastasis; glycolipids; sialyltransferase; glycosyltransferase;
D O I
10.1006/abbi.2001.2320
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have determined that the production of a metastasis-associated neutral glycosphingolipid, isogloboside (iGb(4)Cer, GalNAc beta1-3Gal alpha1-3Gal beta1-4Glc beta1-O-ceramide) is associated with the loss of G(M3) synthase activity. Assays for neutral glycosphingolipid-forming glycosyltransferases in cells producing various levels of iGb(4)Cer revealed no consistent differences that could account for the difference in iGb(4)Cer biosynthesis. However, comparison of the activity of G(M3) synthase in homogenates of these two cell types revealed that cells that did not synthesize iGb(4)Cer had activity significantly greater than that of cells possessing this antigen, Furthermore, somatic cell. hybrids generated using clones of the iGb(4)Cer -producing and nonproducing cell lines lacked iGb(4)Cer while possessing high levels of G(M3) synthase activity. When iGb(4)Cer-producing cells were transfected with a G(M3) synthase expression vector, all of the resultant clones were negative for iGb(4)Cer production, The results of these studies clearly show that the presence of G(M3) synthase prevents the formation of iGb(4)Cer in these cells. (C) 2001 Academic Press.
引用
收藏
页码:187 / 194
页数:8
相关论文
共 42 条
[1]  
[Anonymous], 1977, Lipids, V12, P455
[2]   HYGROMYCIN-B PHOSPHOTRANSFERASE AS A SELECTABLE MARKER FOR DNA TRANSFER EXPERIMENTS WITH HIGHER EUKARYOTIC CELLS [J].
BLOCHLINGER, K ;
DIGGELMANN, H .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (12) :2929-2931
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]  
BUCKLEY ND, 1988, CANCER RES, V48, P1451
[5]  
BURCZAK JD, 1988, BIOCHIM BIOPHYS ACTA, V804, P442
[6]  
CARLSEN SA, 1984, CANCER RES, V44, P3012
[7]   THE IDENTIFICATION OF A NEUTRAL GLYCOSPHINGOLIPID ANTIGENIC MARKER FOR METASTATIC CELLS IN THE R3230AC RAT MAMMARY ADENOCARCINOMA [J].
CARLSEN, SA ;
BARRY, M ;
NEWTON, K .
CLINICAL & EXPERIMENTAL METASTASIS, 1990, 8 (02) :141-151
[8]  
CARLSEN SA, 1993, CANCER RES, V44, P3012
[9]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[10]   BETA-1-6 BRANCHING OF ASN-LINKED OLIGOSACCHARIDES IS DIRECTLY ASSOCIATED WITH METASTASIS [J].
DENNIS, JW ;
LAFERTE, S ;
WAGHORNE, C ;
BREITMAN, ML ;
KERBEL, RS .
SCIENCE, 1987, 236 (4801) :582-585