Regulation of osteoclast protease expression by RANKL

被引：56

作者：

Wittrant, Y ^{[1
]}

Theoleyre, S ^{[1
]}

Couillaud, S ^{[1
]}

Dunstan, C ^{[1
]}

Heymann, D ^{[1
]}

Rédini, F ^{[1
]}

机构：

[1] Fac Med, Lab Physiopathol Resorpt Osseuse & Therapie Tumeu, F-44035 Nantes 1, France

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 310卷 / 03期

关键词：

RANKL; osteoclasts; bone resorption; metalloproteinase;

D O I：

10.1016/j.bbrc.2003.09.084

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Receptor activator of NF-kappaB ligand (RANKL) is essential for osteoclast (OC) differentiation/activation and functions through its receptor RANK at the surface of the osteoclastic cells. This study investigated for the first time the direct effects of hRANKL on protease/protease inhibitor expressions and protease activities in purified rabbit osteoclast cultures, using semi-quantitative RTPCR, gelatin zymography, and enzymatic assays. RANKL was shown to exert in vitro pro-resorptive effects by increasing osteoclast marker expressions (Tartrate resistant acid phosphatase (TRAP) and cathepsin K), MMP-9 expression, and pro-MMP-9 activity and by diminishing TIMP-1 expression, leading to an up-regulation of the MMP-9/TIMP-1 ratio. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：774 / 778

页数：5

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