Electrogenic properties of the Na+,K+-ATPase probed by presteady state and relaxation studies

被引：6

作者：

Bamberg, E

Clarke, RJ

Fendler, K

机构：

[1] Max Planck Inst Biophys, D-60596 Frankfurt, Germany

[2] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2001年 / 33卷 / 05期

关键词：

BLM; patch clamp; rate constant; access channel; sodium pump; transport;

D O I：

10.1023/A:1010667407003

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Electrical measurements on planar lipid bilayers, patch/voltage clamp experiments, and spectroscopic investigations involving a potential sensitive dye are reviewed. These experiments were performed to analyze the kinetics of charge translocation of the Na+,K+-ATPase. High time resolution was achieved by applying caged ATP, voltage-jump, and stopped-flow techniques, respectively. Kinetic parameters and the electrogenicity of the relevant transitions in the Na+,K+-ATPase reaction cycle are discussed.

引用

收藏

页码：401 / 405

页数：5

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