Investigation of microcontact transfer of proteins from a selectively plasma treated elastomer stamp by fluorescence microscopy and force microscopy

被引：5

作者：

Feng, XZ ^{[1
]}

Roberts, CJ ^{[1
]}

Armitage, DA ^{[1
]}

Davies, MC ^{[1
]}

Tendler, SJB ^{[1
]}

Allen, S ^{[1
]}

Williams, PM ^{[1
]}

机构：

[1] Univ Nottingham, Dept Pharmaceut Sci, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England

来源：

ANALYST | 2001年 / 126卷 / 07期

关键词：

D O I：

10.1039/b102269p

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Selective plasma treatment of the recessed regions of the elastomer stamps is shown to alter the resultant protein patterns. Fluorescence microscopy is demonstrated to be an excellent tool to discriminate between regions of microcontact printed fluorescent dye-labelled albumin in polystyrene. Atomic force microscopy and shear force microscopy are used to provide high-resolution images of the patterned protein layers. The formation and characteristics of the patterns formed by these alternative strategies is discussed.

引用

页码：1100 / 1104

页数：5

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