Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging

被引:121
作者
Zhang, JY
Richards, LJ
Yarowsky, P
Huang, H
van Zijl, PCM
Mori, S
机构
[1] Johns Hopkins Univ, Sch Med, Dept Radiol, Div MRI Res, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA
[3] Univ Maryland, Sch Med, Dept Anat & Neurobiol, Baltimore, MD 21201 USA
[4] Program Neurosci, Baltimore, MD 21201 USA
[5] Univ Maryland, Sch Med, Dept Pharmacol & Expt Therapeut, Baltimore, MD 21201 USA
[6] Kennedy Krieger Inst, FM Kirby Res Ctr Funct Brain Imaging, Baltimore, MD 21205 USA
关键词
D O I
10.1016/S1053-8119(03)00410-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Investigation of three-dimensional (3D) morphometry of developing brains has been hindered by a lack of imaging modalities that can monitor the 3D evolution of various anatomical structures without sectioning and staining processes. In this study, we combined magnetic resonance microimaging and diffusion tensor imaging techniques to accomplish such visualization. The application of this approach to developing mouse embryos revealed that it could clearly delineate early critical structures such as neuroepithelium, cortical plate, and various axonal structures, and follow their developmental evolution. The technique was applied to the study of the Netrin-1 mutant, allowing verification of its anatomical phenotype. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:1639 / 1648
页数:10
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