Maternal cytokine production patterns in women with pre-eclampsia

被引:95
作者
Azizieh, F
Raghupathy, R
Makhseed, M
机构
[1] Kuwait Univ, Fac Med, Dept Microbiol, Safat 13110, Kuwait
[2] Kuwait Univ, Fac Med, Dept Obstet & Gynecol, Safat 13110, Kuwait
关键词
pre-eclampsia; Th1; cytokines; Th2;
D O I
10.1111/j.1600-0897.2005.00278.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
PROBLEM: To determine the levels of cytokines produced upon mitogenic or antigenic stimulation of maternal peripheral blood mononuclear cells (PBMC) from women with pre-eclampsia. METHOD OF STUDY: PBMC from 54 women with a history of successful pregnancy and 32 women undergoing pre-eclamptic delivery were stimulated with a mitogen or with autologous placental cells or with trophoblast antigens, and the levels of cytokines released into the culture supernatants then assessed by enzyme-linked immunosorbent assay. RESULTS: Significantly higher levels of the Th1 cytokines, interferon-gamma, and tumor necrosis factor-ot were produced by the pre-eclamptic group than by the normal pregnancy group, which on the contrary showed significantly greater production of the Th2 cytokines, interleukin (IL)-4, IL-5, IL-6 and IL-10. A comparison of the ratios Of Th2 to Th1 cytokines indicates a higher Th1 cytokine bias in pre-eclampsia as compared with normal pregnancy. CONCLUSIONS: These data are suggestive of a maternal proinflammatory cytokine bias in pre-eclampsia.
引用
收藏
页码:30 / 37
页数:8
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