mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules

被引：81

作者：

Masuda, S ^{[1
]}

Saito, H ^{[1
]}

Nonoguchi, H ^{[1
]}

Tomita, K ^{[1
]}

Inui, K ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 3,KUMAMOTO 860,JAPAN

来源：

FEBS LETTERS | 1997年 / 407卷 / 02期

关键词：

organic anion transporter; methotrexate; microdissection; brush-border membrane; rat kidney;

D O I：

10.1016/S0014-5793(97)00314-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolocalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules, Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney, These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules. (C) 1997 Federation of European Biochemical Societies.

引用

页码：127 / 131

页数：5

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