The antibacterial activity against MRSA strains and other bacteria of a <500 Da fraction from maggot excretions/secretions of Lucilia sericata (Diptera: Calliphoridae)

被引：83

作者：

Bexfield, Alyson ^{[1
]}

Bond, A. Elizabeth ^{[1
,2
]}

Roberts, Emily C. ^{[1
]}

Dudley, Edward ^{[1
,2
]}

Nigam, Yamni ^{[3
]}

Thomas, Stephen ^{[4
]}

Newton, Russell P. ^{[1
,2
]}

Ratcliffe, Norman A. ^{[1
]}

机构：

[1] Swansea Univ, Dept Biol Sci, SOTEAS, Swansea SA2 8PP, W Glam, Wales

[2] Swansea Univ, BAMS Facil, Swansea SA2 8PP, W Glam, Wales

[3] Swansea Univ, Sch Hlth Sci, Swansea SA2 8PP, W Glam, Wales

[4] Medetec Med Device Tech Consultancy Serv, Cardiff, S Glam, Wales

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 04期

关键词：

maggot therapy; MRSA; antibacterial; bacterial morphology;

D O I：

10.1016/j.micinf.2007.12.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

The application of Lucilia sericata larvae to chronic, infected wounds results in the rapid elimination of infecting microorganisms, including MRSA. Previously, we demonstrated in vitro antibacterial activity of native excretions/secretions (nES) from L. sericata and partially purified two low mass antibacterial compounds with masses of 0.5-10 kDa and <500 Da. The present study reports the antibacterial effects of the <500 Da fraction (ES<500) on the growth and morphology of a range of bacteria, including 12 MRSA strains. Distinct morphological changes were observed in Bacillus cereus and Escherichia coli following exposure to ES<500. Flow cytometry and confocal microscopy analyses, in conjunction with turbidometric and CFU assays, revealed bacteriostatic activity of nES against S. aureus and E. coli. ES1500 also demonstrated bacteriostatic activity against S. aureus, however, bactericidal activity and the induction of a viable but non-culturable state were observed with ES<500-treated E. coli. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：325 / 333

页数：9

共 31 条

[1]

Temperature shift experiments with ftsZ84(Ts) strain reveal rapid dynamics of FtsZ localization and indicate that the Z ring is required throughout septation and cannot reoccupy division sites once constriction has initiated [J].

Addinall, SG ;

Cao, C ;

Lutkenhaus, J .

JOURNAL OF BACTERIOLOGY, 1997, 179 (13) :4277-4284

[2]

[Anonymous], 2002, MMWR MORB MORTAL WKL, V51, P565

[3]

Detection and partial characterisation of two antibacterial factors from the excretions/secretions of the medicinal maggot Lucilia sericata and their activity against methicillin-resistant Staphylococcus aureus (MRSA) [J].

Bexfield, A ;

Nigam, Y ;

Thomas, S ;

Ratcliffe, NA .

MICROBES AND INFECTION, 2004, 6 (14) :1297-1304

[4]

Bowler PG, 1999, WOUNDS, V11, P72

[5]

Dysregulation of bacterial proteolytic machinery by a new class of antibiotics [J].

Brötz-Oesterhelt, H ;

Beyer, D ;

Kroll, HP ;

Endermann, R ;

Ladel, C ;

Schroeder, W ;

Hinzen, B ;

Raddatz, S ;

Paulsen, H ;

Henninger, K ;

Bandow, JE ;

Sahl, HG ;

Labischinski, H .

NATURE MEDICINE, 2005, 11 (10) :1082-1087

[6]

ELECTRON MICROSCOPY OF PROTEUS VULGARIS EXPOSED TO CEPHALOTHIN [J].

BURDASH, NM ;

EHRLICH, MA ;

EHRLICH, HG ;

PARISI, JT .

JOURNAL OF BACTERIOLOGY, 1968, 95 (05) :1956-&

[7]

CASEWELL MW, 1986, J ANTIMICROB CHEMOTH, V18, P1

[8]

MORPHOLOGICAL CHANGES IN GRAM-NEGATIVE BACILLI EXPOSED TO CEPHALOTHIN [J].

CHANG, TW ;

WEINSTEIN, L .

JOURNAL OF BACTERIOLOGY, 1964, 88 (06) :1790-&

[9]

Courtenay M, 1999, J Wound Care, V8, P177

[10]

Mortality from methicillin resistant Staphylococcus aureus in England and Wales:: analysis of death certificates [J].

Crowcroft, NS ;

Catchpole, M .

BRITISH MEDICAL JOURNAL, 2002, 325 (7377) :1390-1391

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The antibacterial activity against MRSA strains and other bacteria of a &lt;500 Da fraction from maggot excretions/secretions of Lucilia sericata (Diptera: Calliphoridae)

The antibacterial activity against MRSA strains and other bacteria of a <500 Da fraction from maggot excretions/secretions of Lucilia sericata (Diptera: Calliphoridae)