A direct glimpse of cross-hybridization: Background-passified microarrays that allow mass-spectrometric detection of captured oligonucleotides

被引：19

作者：

Plutowski, U ^{[1
]}

Richert, C ^{[1
]}

机构：

[1] Univ Karlsruhe, Inst Organ Chem, D-76131 Karlsruhe, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 04期

关键词：

cross-hybridization; DNA recognition; mass spectrometry; oligonucleoticles; self-assembled monolayers;

D O I：

10.1002/anie.200461212

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oligonucleotides immobilized in SAMs (self-assembled monolayers) with a passifying surface layer, prepared from thermally smoothed gold surfaces and disulfides, allow the direct detection of bound strands by MALDI-TOF mass spectrometry. The binding of perfectly matched (PM) and mismatched (MM) DNA targets is observed (see picture). The extent to which cross-hybridization occurs depends on the type and position of the mismatch.

引用

页码：621 / 625

页数：5

共 31 条

[1] In situ synthesis of oligonucleotide arrays by using surface tension [J].

Butler, JH ;

Cronin, M ;

Anderson, KM ;

Biddison, GM ;

Chatelain, F ;

Cummer, M ;

Davi, DJ ;

Fisher, L ;

Frauendorf, AW ;

Frueh, FW ;

Gjerstad, C ;

Harper, TF ;

Kernahan, SD ;

Long, DQ ;

Pho, M ;

Walker, JA ;

Brennan, TM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (37) :8887-8894

[2] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[3] 5′-Tethered stilbene derivatives as fidelity- and affinity-enhancing modulators of DNA duplex stability [J].

Dogan, Z ;

Paulini, R ;

Stütz, JAR ;

Narayanan, S ;

Richert, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (15) :4762-4763

[4]

Dombi KL, 2002, SYNTHESIS-STUTTGART, P816

[5] Hybridization cross-reactivity within homologous gene families on glass cDNA microarrays [J].

Evertsz, EM ;

Au-Young, J ;

Ruvolo, MV ;

Lim, AC ;

Reynolds, MA .

BIOTECHNIQUES, 2001, 31 (05) :1182-+

[6] LIGHT-DIRECTED, SPATIALLY ADDRESSABLE PARALLEL CHEMICAL SYNTHESIS [J].

FODOR, SPA ;

READ, JL ;

PIRRUNG, MC ;

STRYER, L ;

LU, AT ;

SOLAS, D .

SCIENCE, 1991, 251 (4995) :767-773

[7] Sequence context and thermodynamic stability of a single base pair mismatch in short deoxyoligonucleotide duplexes [J].

Hall, TS ;

Pancoska, P ;

Riccelli, PV ;

Mandell, K ;

Benight, AS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (47) :11811-11812

[8] Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds [J].

Hong, R ;

Fischer, NO ;

Verma, A ;

Goodman, CM ;

Emrick, T ;

Rotello, VM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (03) :739-743

[9] Summaries of affymetrix GeneChip probe level data [J].

Irizarry, RA ;

Bolstad, BM ;

Collin, F ;

Cope, LM ;

Hobbs, B ;

Speed, TP .

NUCLEIC ACIDS RESEARCH, 2003, 31 (04) :e15

[10] Electrochemical desorption of self-assembled monolayers noninvasively releases patterned cells from geometrical confinements [J].

Jiang, XY ;

Ferrigno, R ;

Mrksich, M ;

Whitesides, GM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (09) :2366-2367

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