Application of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-(dione to analysis of 25-hydroxyvitamin D3 in human plasma by liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry

被引：30

作者：

Higashi, T ^{[1
]}

Yamauchi, A ^{[1
]}

Shimada, K ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

来源：

ANALYTICAL SCIENCES | 2003年 / 19卷 / 06期

关键词：

D O I：

10.2116/analsci.19.941

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The utility of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione (NPTAD) as a derivatization reagent in the analysis of 25-hydroxyvitamin D-3 [25(OH)D-3] using liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry (LC/ECAPCI-MS) was examined. The derivatives of 25(OH)D-3 with NPTAD underwent electron capture in the APCI source in the negative-ion mode and provided 30-fold higher sensitivity compared to an intact compound. This derivatization-LC/ECAPCI-MS method was applied to a plasma 25-hydroxyvitamin D-3 assay, and gave satisfactory results in sensitivity, specificity, measurable range and throughput of the analysis.

引用

收藏

页码：941 / 943

页数：3

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