Highly sensitive method for the determination of melatonin by normal-phase high-performance liquid chromatography with fluorometric detection

被引：43

作者：

Vitale, AA ^{[1
]}

Ferrari, CC ^{[1
]}

Aldana, H ^{[1
]}

Affanni, JM ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT CIENCIAS BIOL,RA-1428 BUENOS AIRES,DF,ARGENTINA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 681卷 / 02期

关键词：

melatonin;

D O I：

10.1016/0378-4347(96)00051-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study a new chromatographic method was developed to quantify melatonin in rat pineal that can be extended to other tissues. Melatonin was extracted from an acid homogenate with ethyl acetate to avoid amine interference. HPLC was performed with silica normal-phase column and fluorescence detection. This method is sensitive enough for detecting melatonin in a single pineal gland with a detection limit of 3 pg/mg tissue.

引用

页码：381 / 384

页数：4

共 28 条

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