Th1 and Th17 Cytokines Drive Inflammation in Takayasu Arteritis

被引：222

作者：

Saadoun, D. ^{[1
,2
]}

Garrido, M. ^{[1
,2
]}

Comarmond, C. ^{[1
,2
]}

Desbois, A. C. ^{[1
,2
]}

Domont, F. ^{[1
,2
]}

Savey, L. ^{[1
,2
]}

Terrier, B. ^{[1
,2
]}

Geri, G. ^{[1
,2
]}

Rosenzwajg, M. ^{[1
,2
]}

Klatzmann, D. ^{[1
,2
]}

Fourret, P. ^{[3
,4
]}

Cluzel, P. ^{[3
,4
]}

Chiche, L. ^{[3
,4
]}

Gaudric, J. ^{[3
,4
]}

Koskas, F. ^{[3
,4
]}

Cacoub, P. ^{[1
,2
]}

机构：

[1] Univ Paris 06, CNRS, Grp Hosp Pitie Salpetriere, UMR 7211,INSERM,U959, Paris, France

[2] Ctr Natl Reference Malad Autoimmunes & Syst Rares, Paris, France

[3] Grp Hosp Pitie Salpetriere, F-75634 Paris, France

[4] Univ Paris 06, Paris, France

来源：

ARTHRITIS & RHEUMATOLOGY | 2015年 / 67卷 / 05期

关键词：

LARGE-VESSEL VASCULITIS; GIANT-CELL ARTERITIS; AORTIC TISSUE; DISEASE; INTERLEUKIN-6; PROTEIN;

D O I：

10.1002/art.39037

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Objective. Takayasu arteritis (TAK) is a large-vessel vasculitis that induces damage to the aorta and its branches. Glucocorticoids remain the gold standard of therapy for TAK. The nature of the T cells driving vascular inflammation and the effects of glucocorticoids on the systemic components of TAK are not understood. The aim of this study was to analyze T cell homeostasis and cytokine production in peripheral blood and inflammatory lesions of the aorta in patients with TAK. Methods. T cell homeostasis and cytokine production in peripheral blood and inflammatory lesions of the aorta were analyzed using Luminex analysis, flow cytometry, and immunohistochemical analysis. The study included 41 patients fulfilling the American College of Rheumatology 1990 criteria for the classification of TAK (17 patients with active TAK and 24 patients with disease in remission), 30 patients with giant cell arteritis and 39 patients with Behcet's disease (disease controls), and 20 age- and sex-matched healthy control subjects. Results. We observed a marked increase in the expression of Th1 and Th17 cells, which correlated with TAK disease activity. The addition of serum from patients with active TAK to sorted CD41 T cells from healthy donors in culture medium induced significant production of interferon-gamma (IFN gamma) and interleukin-17A (IL-17A). We demonstrated the presence of IFN gamma-, IL-6-, and IL-17A-producing T cells in vascular inflammatory infiltrates in patients with TAK. Corticosteroid therapy was associated with decreased levels of circulating Th1 cytokines in corticosteroid-treated patients with TAK compared with steroid-free patients with TAK (for IL-2, mean +/- SD 5,079 +/- 5,300 versus 7,359 +/- 3,197 pg/ml; for IFN gamma, 2,592 +/- 3,072 versus 8,393 +/- 3,392 pg/ml; for tumor necrosis factor alpha, 847 +/- 724 versus 1,491 +/- 392 pg/ml). However, glucocorticoids had essentially no effect on the frequency of Th17 cytokines (IL-1 receptor, IL-17, and IL-23). Conclusion. The Th17 and Th1 pathways contribute to the systemic and vascular manifestations of TAK. Glucocorticoid treatment suppresses Th1 cytokines but spares Th17 cytokines in patients with TAK.

引用

页码：1353 / 1360

页数：8

共 18 条

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[2]

The development of inflammatory TH-17 cells requires interferon-regulatory factor 4 [J].