Enhanced T cell-independent antibody responses in c-Jun N-terminal kinase 2 (JNK2)-deficient B cells following stimulation with CpG-1826 and anti-IgM

被引：7

作者：

Cao, Yanling ^{[1
,2
]}

Takada, Eiko ^{[1
,2
]}

Hata, Kikumi ^{[1
,2
]}

Sudo, Katsuko ^{[3
]}

Furuhata, Masae ^{[1
,2
]}

Mizuguchi, Junichiro ^{[1
,2
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Immunol, Shinjuku Ku, Tokyo 1608402, Japan

[2] Tokyo Med & Dent Univ, Intractable Immunol Res Ctr, Shinjuku Ku, Tokyo 1608402, Japan

[3] Tokyo Med & Dent Univ, Anim Res Ctr, Shinjuku Ku, Tokyo 1608402, Japan

来源：

IMMUNOLOGY LETTERS | 2010年 / 132卷 / 1-2期

关键词：

JNK2; B cells; Antibody responses; T cell-independent; CpG; TOLL-LIKE RECEPTORS; CLASS SWITCH RECOMBINATION; SIGNAL-TRANSDUCTION; INDUCED APOPTOSIS; PROTEIN-KINASES; UP-REGULATION; ACTIVATION; TLR9; JNK; EXPRESSION;

D O I：

10.1016/j.imlet.2010.05.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Although c-Jun NH2-terminal kinase (JNK) 1 and JNK2 have been demonstrated to modulate T cell activation, role of JNKs in B cell activation remains largely unclear. Phosphorylation of JNK2 was increased in murine B cells following stimulation with either anti-IgM or CpG-1826 oligonucleotide (ODN) alone, with a further increase by a combined stimulation with anti-IgM and CpG-1826 ODN. In this study, we examined whether antibody production induced by CpG ODN and/or anti-IgM is affected in B cells from JNK2-deficient (JNK2(-/-)) mice. After stimulation with CpG ODN or both CpG ODN and anti-IgM, JNK2(-/-) B cells displayed an enhanced antibody production of IgG1 and IgG2a, with less pronounced in IgG2b production, as assessed by enzyme-linked immunoassay (ELISA). However, IgM production in JNK2(-/-) cells by CpG ODN was comparable to that in WT B cells. TLR9 expression was increased in JNK2(-/-) B cells after stimulation with anti-IgM or both CpG ODN and anti-IgM, suggesting that the anti-IgM/CpG ODN-induced enhancement of antibody production is partly due to the increased expression of TLR9. The enhanced antibody production in JNK2(-/-) B cells by the combined stimulation does not appear to involve either increased class switch recombination or cell proliferation. Our results provide useful information on the role of JNK2 in antibody responses mediated by T cell-independent antigens. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：38 / 44

页数：7

共 39 条

[1] Toll-like receptor signalling [J].

Akira, S ;

Takeda, K .

NATURE REVIEWS IMMUNOLOGY, 2004, 4 (07) :499-511

[2] A role for Toll-like receptors in acquired immunity: up-regulation of TLR9 by BCR triggering in naive B cells and constitutive expression in memory B cells [J].

Bernasconi, NL ;

Onai, N ;

Lanzavecchia, A .

BLOOD, 2003, 101 (11) :4500-4504

[3] The CD40-CD154 interaction in B cell-T cell liaisons [J].

Bishop, GA ;

Hostager, BS .

CYTOKINE & GROWTH FACTOR REVIEWS, 2003, 14 (3-4) :297-309

[4] The toll-like receptor repertoire of human B lymphocytes: inducible and selective expression of TLR9 and TLR10 in normal and transformed cells [J].

Bourke, E ;

Bosisio, D ;

Golay, J ;

Polentarutti, N ;

Mantovani, A .

BLOOD, 2003, 102 (03) :956-963

[5]

CAO Y, 2010, J TOKYO MED U, V68, P45

[6] Mammalian MAP kinase signalling cascades [J].

Chang, LF ;

Karin, M .

NATURE, 2001, 410 (6824) :37-40

[7] MAP kinases in the immune response [J].

Dong, C ;

Davis, RJ ;

Flavell, RA .

ANNUAL REVIEW OF IMMUNOLOGY, 2002, 20 :55-72

[8] Defective T cell differentiation in the absence of Jnk1 [J].

Dong, C ;

Yang, DD ;

Wysk, M ;

Whitmarsh, AJ ;

Davis, RJ ;

Flavell, RA .

SCIENCE, 1998, 282 (5396) :2092-2095

[9] Activation-induced cell death in B lymphocytes [J].

Donjerkovic, D ;

Scott, DW .

CELL RESEARCH, 2000, 10 (03) :179-192

[10] BCR-mediated uptake of antigen linked to TLR9 ligand stimulates B-cell proliferation and antigen-specific plasma cell formation [J].

Eckl-Dorna, Julia ;

Batista, Facundo D. .

BLOOD, 2009, 113 (17) :3969-3977

← 1 2 3 4 →