Dispase detergent treated dermal matrix as a dermal substitute

被引：123

作者：

Takami, Y ^{[1
]}

Matsuda, T ^{[1
]}

Yoshitake, M ^{[1
]}

Hanumadass, M ^{[1
]}

Walter, RJ ^{[1
]}

机构：

[1] COOK CTY HOSP,HEKTOEN INST MED RES,DEPT SURG,CHICAGO,IL 60612

来源：

BURNS | 1996年 / 22卷 / 03期

关键词：

D O I：

10.1016/0305-4179(95)00123-9

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

A method for preparing acellular allogeneic dermal matrix (ADM) and ifs effectiveness as a dermal substitute are described. Treatment of rat skin with Dispase followed by Triton X-100 completely removed cellular components from the dermis. Subcutaneously implanted ADM evoked no immunological reaction and 20 weeks after implantation, the size of the implanted ADM was reduced to about 60 per cent of its original area. ADM became completely vascularized within 2 weeks after implantation into full thickness skin defects in the rat and inhibited extensive wound contracture. A second layer of ADM placed onto the implanted ADM served as an excellent dressing, providing mechanical protection and permitting vascularization of the underlying implant. Onlay skin autografts placed onto vascularized allogeneic ADM showed good survival when the skin was grafted more than I week after ADM implantation. Dispase/detergent treated ADM derived front animal or human skin may be useful in fall thickness skin defects providing a vascularized bed for subsequent epidermal coverage.

引用

页码：182 / 190

页数：9

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