SELECTIVE CULTURE OF DIFFERENT TYPES OF HUMAN PAROTID GLAND CELLS

被引：7

作者：

Chan, Yen-Hui ^{[1
,4
]}

Huang, Tsung-Wei ^{[1
,2
,3
,4
]}

Young, Tai-Horng ^{[1
,4
]}

Lou, Pei-Jen ^{[5
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Inst Biomed Engn, Taipei 10764, Taiwan

[2] Far Eastern Mem Hosp, Dept Otolaryngol, Taipei, Taiwan

[3] Oriental Inst Technol, Dept Hlth Care Ministrat, Taipei, Taiwan

[4] Natl Taiwan Univ, Coll Engn, Taipei 10764, Taiwan

[5] Natl Taiwan Univ Hosp, Dept Otolaryngol, Taipei, Taiwan

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2011年 / 33卷 / 03期

关键词：

human parotid gland; primary culture; acinar cells; myoepithelial cells; immunocytochemistry; ARTIFICIAL SALIVARY-GLAND; EPITHELIAL-CELLS; MYOEPITHELIAL CELLS; IN-VITRO; TISSUE; CULTIVATION; PHENOTYPE; GROWTH; LINE;

D O I：

10.1002/hed.21465

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 [耳鼻咽喉科学];

摘要：

Background. Advances in salivary gland tissue engineering can benefit patients diagnosed with xerostomia. Complexity of the gland explains the urgent demand for a reliable protocol to isolate and expand various gland cells that can be used for further study. Methods. Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level. Results. Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique. Conclusion. The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies. (C) 2010 Wiley Periodicals, Inc. Head Neck 33: 407-414, 2011

引用

页码：407 / 414

页数：8

共 22 条

[1]

Myoepithelial cells: good fences make good neighbors [J].

Adriance, MC ;

Inman, JL ;

Petersen, OW ;

Bissell, MJ .

BREAST CANCER RESEARCH, 2005, 7 (05) :190-197

[2]

Current status of the development of an artificial salivary gland [J].

Aframian, Doron J. ;

Palmon, Aaron .

TISSUE ENGINEERING PART B-REVIEWS, 2008, 14 (02) :187-198

[3]

Blatchford DR, 1999, J CELL PHYSIOL, V181, P304, DOI 10.1002/(SICI)1097-4652(199911)181:2<304::AID-JCP12>3.0.CO

[4]

2-5

[5]

BURFORDMASON AP, 1994, LARYNGOSCOPE, V104, P335

[6]

SECRETION OF ALPHA-AMYLASE IN HUMAN PAROTID-GLAND EPITHELIAL-CELL CULTURE [J].

CHOPRA, DP ;

XUEHU, IC .

JOURNAL OF CELLULAR PHYSIOLOGY, 1993, 155 (02) :223-233

[7]

Isolation and cultivation of integrin α6β1-expressing salivary gland graft cells:: A model for use with an artificial salivary gland [J].

David, Ran ;

Shai, Ela ;

Aframian, Doron J. ;

Palmon, Aaron .

TISSUE ENGINEERING PART A, 2008, 14 (02) :331-337

[8]

Gudjonsson T, 2002, J CELL SCI, V115, P39

[9]

Calcium induces differentiation of primary human salivary acinar cells [J].

Hiraki, A ;

Shirasuna, K ;

Ikari, T ;

Shinohara, M ;

Garrod, DR .

JOURNAL OF CELLULAR PHYSIOLOGY, 2002, 193 (01) :55-63

[10]

Hoffman MP, 1996, J CELL SCI, V109, P2013

← 1 2 3 →